重组人类骨形态蛋白2复合自固化磷酸钙材料在体内的血管化  

Vascularization of autosetting calcium phosphate cultivated with recombinant human bone morphogenetic protein 2

作　　者：王炜[1] 玛依拉.吾甫尔 阿不都赛米.艾买提 艾合买提江.玉素甫[1] 

机构地区：[1]新疆医科大学第一附属医院骨科中心显微修复外科,新疆维吾尔自治区乌鲁木齐市830054 [2]新疆医科大学第一附属医院心脏中心,新疆维吾尔自治区乌鲁木齐市830054

出　　处：《中国组织工程研究与临床康复》2011年第29期5359-5362,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(30960390);新疆医科大学一附院科研专项基金(2007-GXB-04)资助~~

摘　　要：背景:深筋膜瓣促进组织工程骨血管化是一种成熟的技术,但动物种属不同、植入材料不同均会使血管化的结果产生较大的差异。目的:比较复合重组人类骨形态蛋白2的自固化磷酸钙人工骨和单纯的自固化磷酸钙人工骨在比格犬带蒂筋膜瓣内的血管再生能力。方法:分别将复合重组人骨形态蛋白2的自固化磷酸钙人工骨和单纯的自固化磷酸钙人工骨包裹于12只成年比格犬腰背部两侧带蒂深筋膜瓣中,于术后第2,4,8,16周各随机选取3只动物摘取血管化标本,进行大体观察、苏木精-伊红染色、Masson染色及血管内皮细胞Ⅷ因子相关抗原(ⅧRAg)免疫组织化学染色观察比较新血管的生成情况。结果与结论:在材料植入后4周、8周和16周,两组均发现带蒂筋膜与植入材料接触区出现明显血管化,并随时间延长,向材料内部延伸;实验组相对血管面积百分比和阳性染色的面密度值在2,4,8,16周时均高于对照组。结果显示两种人工骨在筋膜内均有一定的血管化能力,但复合重组人骨形态蛋白2的自固化磷酸钙人工松质骨在体内的血管化较单纯自固化磷酸钙人工松质骨更为明显。BACKGROUND： Deep fascia promotes vascularization of tissue-engineered bone, which is a mature technology, but different animal species and different implant materials can result in a great difference in vascularization. OBJECTIVE： To compare the vascularization of autosetting calcium phosphate cement cultivated with recombinant human bone morphogenetic protein 2 （rhBMP-2） and a single autosetting calcium phosphate cement implant in beagle dogs with deep fasica flaps. METHODS： Twelve healthy adult beagle dogs were respectively implanted with autosetting calcium phosphate cement cultivated with rhBMP-2 in the left thigh （experimental sides） and a single autosetting calcium phosphate cement in right thigh （control sides）. The vascularization in each condition was assessed by experiment study （Physical, Massion stain, the hematoxylin-eosin stain, ⅧRAg marked） at time intervals of 2, 4, 8 and 16 weeks after operation. RESULTS AND CONCLUSION： In experimental groups and in control groups, vascularization was found. New vessels invaded in scaffold with time. In experimental groups, the amount of vessels and the expression of ⅧRAg were stronger than those in control groups at 2, 4, 8, and 16 weeks. The deep fasica flaps have great effect on the vascularization. The deep fasica flap binding with rhBMP-2 is proved to be the better in vascularization of autosetting calcium phosphate cement.

关 键 词：血管化 自固化磷酸钙 筋膜瓣 重组人类骨形成蛋白2 比格犬 

分 类 号：R318[医药卫生—生物医学工程]