生肌玉红胶原海绵修复创面损伤  

Sheng Ji Yu Hong collagen matrices for repair of skin wound

作　　者：严婷婷[1] 熊猛[1] 周勇[2] 姚昶[2] 

机构地区：[1]东南大学附属中大医院整形外科,江苏省南京市210009 [2]江苏省中医院普外科,江苏省南京市210009

出　　处：《中国组织工程研究与临床康复》2011年第29期5367-5370,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金项目(30973756);江苏省中医药领军人才项目(LJ200902)~~

摘　　要：背景:生肌玉红胶原海绵能显著促进创面愈合。目的:观察生肌玉红胶原海绵对兔皮肤创面愈合的影响。方法:在新西兰白兔背部制造3处全层皮肤缺损创面,分别以生肌玉红胶原海绵、贝复剂(重组碱性成纤维细胞生长因子外用溶液)、生理盐水修复。观察各组创面愈合时间、创面愈合率,检测创面修复组织内成纤维细胞数、血红素氧化酶1、转化生长因子β1 mRNA与蛋白及增殖细胞核抗原蛋白表达。结果与结论:与贝复剂、生理盐水比较,生肌玉红胶原海绵可显著促进成纤维细胞增殖(P<0.05),增加创面血红素氧化酶1、转化生子因子β1及增殖细胞核抗原的表达(P<0.05),提高创面愈合率(P<0.05),缩短创面愈合时间(P<0.05)。证实生肌玉红胶原海绵能够促进创面修复,其作用机制可能是通过提高创面血红素氧化酶1、转化生长因子β1表达促进细胞增殖,增加成纤维细胞等创面修复细胞。BACKGROUND： Sheng Ji Yu Hong collagen matrices can obviously promote wound healing. OBJECTIVE： To observe the effect of Sheng Ji Yu Hong collagen matrices on rabbit skin wound healing. METHODS： Three full-thickness skin wounds were made and repaired with Sheng Ji Yu Hong collagen matrices, BFGF agent, normal saline solution, separately. Healing time, healing ratio, number of fibroblasts, hemeoxygenase-1 level, transforming growth factor β1 mRNA and protein expression, and proliferating cell nuclear antigen expression were observed. RESULTS AND CONCLUSION： Sheng Ji Yu Hong could promote the proliferation of fibroblasts more remarkably than BFGF agent and normal saline （P 0.05）, increase expressions of hemeoxygenase-1 level, transforming growth factor β1 and proliferating cell nuclear antigen （P 0.05）, elevate healing rate （P 0.05）, and shorten healing time （P 0.05）. Sheng Ji Yu Hong collagen matrices can promote skin wound healing obviously, and the mechanism maybe relates to elevate the expression of hemeoxygenase-1 level and transforming growth factor β1, and preserve the activity of prosthetic cells .

关 键 词：生肌玉红胶原海绵 创面愈合 血红素氧化酶1 转化生长因子Β1 增殖细胞核抗原 

分 类 号：R318[医药卫生—生物医学工程]