纳米Ag-SiO_2导尿管的细胞毒性评价  

Cytotoxicity evaluation of nano-Ag-SiO_2 urethral catheter

作　　者：翟军红[1] 郭云童[1] 刘春[1] 张利[1] 张雁钢[1] 张进[2] 

机构地区：[1]山西医科大学第一医院,山西省太原市030001 [2]山西医科大学,山西省太原市030001

出　　处：《中国组织工程研究与临床康复》2011年第29期5371-5374,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：山西省自然科学基金资助项目(2006011119)~~

摘　　要：背景:具有抗菌活性的纳米Ag-SiO2导尿管可有效降低普通医用导尿管相关性尿路感染的发生率,其物理、化学性质发生很大变化,所以对其安全性需重新评价。目的:比较纳米Ag-SiO2导尿管和普通医用导尿管浸提液对兔尿道上皮细胞的体外细胞活性影响,初步评价纳米Ag-SiO2导尿管的生物安全性。方法:应用机械分离与酶消化法分离培养兔尿道上皮细胞,在体外行扩增后制成细胞悬液,将培养液更换为浸泡纳米Ag-SiO2导尿管及普通导尿管的培养液,采取四甲基偶氮唑盐比色法量化两组浸提液对兔尿道上皮细胞的体外细胞毒性。用酶联免疫检测仪分别测定两组体外细胞的吸光度值,并计算其相对增殖率(RGR),进行毒性评价及比较。结果与结论:纳米Ag-SiO2导尿管和普通医用导尿管均毒性轻微。纳米Ag-SiO2导尿管与普通医用导尿管相对增殖率均数间背景:具有抗菌活性的纳米Ag-SiO2导尿管可有效降低普通医用导尿管相关性尿路感染的发生率,其物理、化学性质发生很大变化,所以对其安全性需重新评价。目的:比较纳米Ag-SiO2导尿管和普通医用导尿管浸提液对兔尿道上皮细胞的体外细胞活性影响,初步评价纳米Ag-SiO2导尿管的生物安全性。方法:应用机械分离与酶消化法分离培养兔尿道上皮细胞,在体外行扩增后制成细胞悬液,将培养液更换为浸泡纳米Ag-SiO2导尿管及普通导尿管的培养液,采取四甲基偶氮唑盐比色法量化两组浸提液对兔尿道上皮细胞的体外细胞毒性。用酶联免疫检测仪分别测定两组体外细胞的吸光度值,并计算其相对增殖率(RGR),进行毒性评价及比较。结果与结论:纳米Ag-SiO2导尿管和普通医用导尿管均毒性轻微。纳米Ag-SiO2导尿管与普通医用导尿管相对增殖率均数间差别无显著性意义(P>0.05)。结果表明,纳米Ag-SiO2导尿管对细胞的生长繁殖同普通医用导尿管一样影响BACKGROUND： Antibacterial activated nano- Ag-SiO2 urethral catheter can reduce the incidence of catheter-associated urinary tract infections obviously. Because of diversification of physical and chemical properties, security shoud be re-evaluated. OBJECTIVE： Based on comparison of nano-Ag-SiO2 urethral catheter and ordinary urethral catheter extracts on in vitro activity of rabbit epithelial cells, to perform a preliminary evaluation of nano-Ag-SiO2 urethral catheter biological safety. METHODS： Mechanical separation and enzymatic digestion were used to isolate and culture rabbit urothelial cells to make cell suspension after in vitro proliferation. The medium was replaced by immersion nano-Ag-SiO2 and general catheter tube medium. MTT assay was performed to quantify two extracts and detect in vitro cytotoxicity of rabbit urethra epithelial cells. Absorbance values of nano-Ag-SiO2 and general urinary catheter groups were measured by enzyme-linked immunosorbent assay to calculate the relative growth rate, and compare and perform toxicity evaluation. RESULTS AND CONCLUSION： Nano-Ag-SiO2 and general anti-infective urinary catheter were slightly toxic. The relative growth rate had no difference between the two groups （P 0.05）. Nano-Ag-SiO2 urethral catheter effect on cell growth and reproduction is same as that of general urinary catheter, and there is no or low cytotoxicity that is consistent with the biological evaluation standards of medical devices.

关 键 词：纳米Ag-SiO2 尿道上皮细胞 导尿管 四甲基偶氮唑盐 安全性 

分 类 号：R318[医药卫生—生物医学工程]