无水甘油保存同种异体肌腱的移植  

Anhydrous glycerol preserved tendon allograft transplantation

作　　者：韩冰[1] 宋一平[1] 王和洪[1] 陈烁[1] 童讯[1] 赵日光[1] 孙燚炎[1] 冯晖[1] 

机构地区：[1]解放军第97医院南京军区创伤修复中心,江苏省徐州市221004

出　　处：《中国组织工程研究与临床康复》2011年第29期5401-5404,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：南京军区医药卫生科研资助项目(06z17)~~

摘　　要：背景:如何处理和保存同种异体肌腱,使之尽可能保留生物活性并降低其免疫原性是近年研究热点。目的:将保存于无水甘油的兔肌腱进行同种异体移植,观察移植后肌腱的组织学变化。方法:将兔肌腱经特殊处理后,常温避光保存在无水甘油中,保存7,12个月后取出肌腱,移植到兔后腿屈肌腱中。饲养3个月后处死并取出移植的肌腱,进行苏木精-伊红染色石蜡切片及透射电镜观察。结果与结论:苏木精-伊红石蜡切片示肌腱纤维排列尚整齐,细胞形态正常,切片某些区域细胞密度较正常组低,未见明显的炎性细胞浸润。透射电镜示肌腱纤维排列整齐,细胞形态正常。结果提示经无水甘油保存的肌腱进行同种异体移植后,在组织学上与正常肌腱相似,无明显的免疫排斥反应。BACKGROUND： How to save tendon allograft so as to retain its biological activity and reduced its immunogenicity has become a focus in recent years. OBJECTIVE： To observe the histological changes following anhydrous glycerol preserved tendon allograft transplantation. METHODS： A special approach was used to process the rabbit tendons, and then the tendons were sealed in anhydrous glycerol at normal atmospheric temperature away from light. The tendons preserved for 7 and 12 months were transplanted into the rabbit flexor tendon in the hind legs. Rabbits were killed after 3 months and the tendon graft was removed. Hematoxylin-eosin staining for paraffin sections as well as the observation of the organizational structure and the morphology of tendons was observed with electron microscope. RESULTS AND CONCLUSION： The hematoxylin-eosin staining showed that tendon fibers arranged neatly, and cell morphology was normal. No significant inflammatory cells were in eyesight. In some certain regions, cell density in the anhydrous glycerol group was lower than that in the normal group. Under the electron microscope, tendon fibers arranged regularly, and the cell morphology was normal. After preservation with anhydrous glycerol, the tendon allograft is similar to normal tendon histologically, and there is no obvious immunological rejection.

关 键 词：肌腱 移植 无水甘油 免疫排斥 日本大耳白兔 

分 类 号：R318[医药卫生—生物医学工程]