重阳木花粉过敏原的分离、纯化和鉴定  

Separation,purification and identification of allergen from Bischofia polycarpa pollen

作　　者：姚敏[1] 刘志刚[2] 孟光[1] 孔小丽[2] 

机构地区：[1]海口市人民医院检验科,海南海口570208 [2]深圳大学过敏反应与免疫学研究所,广东深圳518060

出　　处：《基础医学与临床》2011年第9期1036-1039,共4页Basic and Clinical Medicine

基　　金：国家自然科学基金(30070702);海南省自然科学基金指导性计划(80570)

摘　　要：目的对重阳木花粉变应原蛋白进行分离、纯化和鉴定。方法采用Coca s液提取重阳木花粉的粗提液,通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)分离粗提液蛋白质组分,并测定其分子质量;收集过敏患者血清,用Western blot法鉴定其变应原成分;通过离子交换层析对重阳木花粉变应原进行初步纯化和免疫印迹鉴定。结果分离得到重阳木花粉18条蛋白带,其中分子质量为12和14 ku的是重阳木花粉的特异性变应原,通过离子交换柱层析方法纯化得到其相应的纯化蛋白。结论对重阳木花粉变应原进行了初步的分离、纯化和鉴定,为临床重阳木花粉过敏疾病的诊断和治疗奠定了基础。Objective To purify and characterize the pollen allergens isolated from Bischofia polycarpa pollen.Methods Pollen extract from Bischofia polycarpa was prepared and analyzed by SDS-PAGE.Antigenic properties of the separated proteins were analyzed by Western blot.Ion-exchange chromatography was used for the purification of the antigens.Then purified products were characterized by Western blot.Results For the pollen extract of Bischofia polycarpa,18 protein bands was found in SDS-PAGE.Two of these protein bands,with a molecular weight of 12 and 14 ku,showed immunoreactivity with IgE from sera of Bischofia polycarpa pollen allergic patients.After the ion-exchange chromatography we got the purified protein.Conclusion Pollen allergens in Bischofia polycarpa pollen were purified and characterized.The result will provide a basis for the diagnosis and treatment of Bischofia polycarpa pollen related allergy.

关 键 词：重阳木花粉 变应原 离子交换柱层析 

分 类 号：R392.12[医药卫生—免疫学]