纤维蛋白诱导的自噬促进人脐静脉内皮细胞增殖与血管腔样结构形成  

Fibrin-induced autophagy promotes cell proliferation and vascule-like formation of HUVECs

作　　者：刘慧慧[1] 曹勇军[1] 张艳林[1] 杨亚萍[1] 刘春风[1] 

机构地区：[1]苏州大学附属第二医院神经内科,215004

出　　处：《江苏医药》2011年第16期1885-1888,共4页Jiangsu Medical Journal

基　　金：苏州市社会发展科技项目(SZD0875)

摘　　要：目的探讨纤维蛋白诱导的自噬对内皮细胞增殖和血管腔样结构形成的作用。方法 MTT法观察在不同浓度和时间的纤维蛋白作用下,人脐静脉内皮细胞(HUVECs)形成血管腔样结构和细胞增殖的改变;采用RT-PCR检测不同浓度(1.5、3.0和6.0mg/ml)和时间(6、12和24h)的纤维蛋白作用下,微管相关蛋白1轻链3(MAP1LC3)和血管内皮生长因子(VEGF)的表达。用自噬抑制剂3-甲基腺嘌呤(3-MA)干预作用最显著的纤维蛋白浓度和时间,观察细胞增殖、血管形成和基因表达变化。结果 HUVECs在纤维蛋白作用下,可见管腔样结构形成,并呈浓度和时间依赖性;细胞增殖也较对照组明显增加。LC3和VEGF的表达均随着纤维蛋白浓度和时间的增加而增加。但在自噬抑制剂3-MA的作用下,细胞增殖降低,未见明显的新生血管形成;并且LC3、VEGF的mRNA表达也明显减少。结论纤维蛋白激活自噬,参与了细胞增殖和血管新生的过程。Objective To investigate the endothelial cells proliferation and vascule-like formation promoted by fibrin-induced autophagy.Methods Under fibrin treatment with varied concentrations（1.5,3.0 and 6.0 mg/ml） and time（6,12 and 24 h）,MTT method was used to detect the changes of cell proliferation and vascule-like formation of human umbilical vein endothelial cells（HUVECs）.Real-time fluracence quantitative-PCR was used to detect the expression of microtubule-associated protein 1 light chain 3（MAP1 LC3） and vascular endotherial growth factor（VEGF） under different concentrations and times of fibrin treatment.Autophagic inhibitor,3-methyladenine（3-MA）,was applied to intervene fibrin-treated endothelial cells and the changes of cell proliferation,angiogenesis and LC3 and VEGF genes expression were observed.Results With fibrin treatment,vasclue-like structure was formed in a dose-and time-dependent manner and cell proliferated significiantly.The expressions of LC3 and VEGF increased with raised concentration and prolonged incubation-time of fibrin treatment.However,intervened with 3-MA,the cell proliferation and angiogenesis were inhibited and the mRNA expressions of LC3 and VEGF were decreased as well.Conclusion Fibrin-induced autophagy involves in the process of cell proliferation and angiogenesis.

关 键 词：纤维蛋白 人脐静脉内皮细胞 自噬 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]