低浓度利福平对鱼藤酮介导的帕金森病细胞模型SH-SY5Y细胞的保护作用  

Protective effects of low concentrations of rifampicin on rotenone-induced SH-SY5Y cells model of Parkinson′s disease

作　　者：钱进军[1] 王颖[1] 赵康仁[1] 吴颉言[1] 张渭芳[1] 王清芳[1] 马瑞[1] 

机构地区：[1]江苏大学第四附属医院神经内科,镇江市212001

出　　处：《江苏医药》2011年第16期1889-1892,共4页Jiangsu Medical Journal

基　　金：江苏省"六大人才高峰"D类资助项目(2007038);江苏省自然科学基金资助项目(BK2008249)

摘　　要：目的探讨低浓度利福平对鱼藤酮诱导的人神经母细胞瘤细胞株(SH-SY5Y)细胞的影响。方法鱼藤酮损伤SH-SY5Y细胞建立帕金森病细胞模型,光学显微镜下观察细胞形态,MTT法检测细胞活性,流式细胞术检测活性氧(ROS)和细胞凋亡率,RT-PCR法检测α-突触核蛋白mRNA。结果经利福平1、10、100nmol/L预处理后,三个利福平组细胞ROS含量、α-突触核蛋白mRNA以及细胞凋亡率均低于鱼藤酮损伤组(P<0.01)。结论利福平具有抗氧化作用,能降低细胞α-突触核蛋白mRNA水平,并对细胞有保护作用。Objective To explore the effects of low concentrations of rifampicin on human neuroblastoma（SH-SY5Y） cells.Methods The cells model of Parkinson′s disease was established by damaging SH-SY5Y cells with rotenone in vitro.Cell morphology was observed by microscope.Cell viability was measured using MTT assay.Intracellular reactive oxygen species（ROS） and apoptosis rate were measured by flow cytometry.α-Synuclein mRNA was measured with RT-PCR.ResultsCompared with rotenone injury control group,ROS,α-synuclein mRNA and apoptosis rate in the groups of pretreated with different concentrations（1,10,100 nmol/L） of rifampicin were significantly decreased（P0.01）.Conclusion Rifampicin has the effects of anti-oxidative stress,reducing the α-synuclein mRNA level,and protecting SH-SY5Y cells from apoptosis.

关 键 词：帕金森病 α-突触核蛋白mRNA 利福平 鱼藤酮 凋亡 

分 类 号：R741[医药卫生—神经病学与精神病学]