地塞米松对人羊膜上皮WISH细胞糖皮质激素受体α表达的影响  

Effects of dexamethasone on glucocorticoid receptor-α expression in human amniotic epithelial cells

作　　者：瞿琳[1] 孙丽洲[1] 纪统慧[1] 

机构地区：[1]南京医科大学第一附属医院妇产科,210029

出　　处：《江苏医药》2011年第16期1916-1919,共4页Jiangsu Medical Journal

摘　　要：目的观察地塞米松对体外培养人羊膜上皮WISH细胞糖皮质激素受体(GR)-α表达的调节。方法将体外培养的WISH细胞分为不同浓度地塞米松(10-8、10-7、10-6、10-5、10-4mol/L)作用48h组,和单一浓度地塞米松作用不同时间组(终浓度10-6mol/L,培养24、487、2h)。两组均设空白对照组。采用RT-PCR方法检测各组细胞中GR-αmRNA的表达。结果在不同浓度地塞米松作用48h后,10-4mol/L组细胞GRα mRNA表达强于空白组,其余各组细胞GR-α mRNA表达均弱于与空白组(P<0.01)。在地塞米松作用不同时间组,各亚组细胞GR-α mRNA表达与空白组相比,均明显减弱(P<0.01)。结论低浓度地塞米松可下调WISH细胞中GR-αmRNA表达;高浓度地塞米松可上调GRα mRNA的表达。GR-α mRNA表达不随地塞米松作用时间的延长而下降。临床上常规剂量和疗程应用地塞米松促胎肺成熟时,不会导致促分娩发动作用。Objective To observe the effects of dexamethasone on glucocorticoid receptor-α（GR-α） expression in the human amniotic epithelial cells,WISH cells.Methods The WISH cells were cultured in vitro with different concentrations of dexamethasone（10-8,10-7,10-6,10-5,10-4mol/L） for 48 h（group A） and a single concentration of dexamethasone（10-6mol/L） for different times of 24,48,and 72 h respectively.The results were compared to the blank control groups.The GR-α mRNA expressions in WISH cells were detected with RT-PCR.Results The expression of GR-α mRNA of WISH cells treated with 10-4mol/L DEX was higher than that of control group,which was loweer in those treated with the other concentration groups.The expression of GR-α mRNA was siginificantly lower in the cells treated with different times than that in those of blank controls（P0.01）.Conclusion The expression of GR-α mRNA is decreased when treated with low concentration of dexamethason（≤10-6mol/L）,which is increased when treated with high concentration of it（≥10-5mol/L）.The decrease of GR-α mRNA expression is not reduced as the treating time prolongs.The clinical dose of dexamethasone for promoting lung paturity of the fetus could not lead to the onset of delivery.

关 键 词：地塞米松 糖皮质激素受体Α 人羊膜上皮细胞 

分 类 号：R714[医药卫生—妇产科学]