甲氨蝶呤对脂多糖诱导大鼠滑膜细胞株RSC-364分泌IL-17和IL-18的影响  

The Effect of Methotrexate on Expression of IL-17 and IL-18 in Rat Synovial Cell Line RSC-364 Stimulated with Lipopolysaccharide(LPS)

作　　者：蔡安季[1] 戴勇 沈化清[1] 蒋莉[1] 苏卓娃[1] 

机构地区：[1]广东医学院附属南山医院检验科,518052 [2]深圳市人民医院临床医学研究中心,518020

出　　处：《放射免疫学杂志》2011年第4期432-433,共2页Journal of Radioimmanology

摘　　要：目的:观察甲氨蝶呤对脂多糖诱导大鼠滑膜细胞株RSC-364分泌IL-17和IL-18表达的影响,进一步探讨甲氨蝶呤治疗类风湿性关节炎(RA)的作用机理。方法:运用ELISA检测不同浓度的甲氨蝶呤对脂多糖诱导大鼠滑膜细胞株RSC-364细胞上清液中的IL-17和-IL-18的含量。结果:甲氨蝶呤明显抑制脂多糖诱导大鼠滑膜细胞株RSC-364细胞分泌炎性因子IL-17和IL-18的含量。结论:甲氨蝶呤抑制脂多糖诱导大鼠滑膜细胞株RSC-364分泌IL-17和IL-18,这可能是其治疗RA的重要的作用机制之一。Objective To investigate the effect of methotrexate（MTX） on expression of IL-17 and IL-18 in the rat synovial cell line RSC-364 stimulated with lipopolysaccharide（LPS） and to illuminate the mechanism of MTX in the treatment of rheumatoid arthritis（RA）.Methods The expression of IL-17 and IL-18 in the rat synvoial cell line RSC-364 stimulated with LPS induced by different doses of MTX were detected by enzyme-linked immunosorbent assays.Results The expression of IL-17 and IL-18 in the rat synvoial cell line RSC-364 stimulated with LPS induced by MTX were obviously suppressed.Conclusion IL-17 and IL-18 in the rat synovial cell line RSC-364 stimulated with LPS were inhibited by MTX,which might be the potential mechanism in the treatment of RA.

关 键 词：甲氨蝶呤 大鼠滑膜细胞株RSC-364 脂多糖 IL-17 IL-18 

分 类 号：R392.11[医药卫生—免疫学]