白细胞介素12对急性放射病小鼠造血系统的影响  

Effect of recombinant murine interleukin 12 on hematopoietic systems in mice of acute radiation sickness

作　　者：王利[1] 王碧薇[1] 赵红霞[1] 左洪莉[1] 赵月莹[1] 余长林[1] 

机构地区：[1]军事医学科学院附属医院,北京100071

出　　处：《国际药学研究杂志》2011年第4期312-316,共5页Journal of International Pharmaceutical Research

基　　金：全军科技攻关课题(NO08g157)

摘　　要：目的研究重组鼠白细胞介素12(rmIL-12)对急性放射病小鼠造血系统的影响。方法 42只BALB/c小鼠均给予6.0 Gy 60Coγ射线全身照射,随机分为照射对照组、5和20μg/kg的rmIL-12治疗组,治疗组分别于照后1 h及此后每3 d一次分别腹腔注射5和20μg/(kg.d)的rmIL-12,共5次,每日2次观察小鼠一般情况,3 d检测1次外周血细胞,分别于照射后14和28 d制备股骨病理切片观察组织形态学改变,收集骨髓细胞进行集落培养。结果 rmIL-12治疗组小鼠一般情况较对照组改善。5和20μg/kg rmIL-12治疗组小鼠外周血中血小板(PLT)数开始恢复时间较对照组均明显提前(分别为11 d和14 d),且PLT最低值均高于对照组(分别为15.9%vs 8.1%,15.1%vs 8.1%,P﹤0.05),28 d时rmIL-12 5和20μg/kg治疗组小鼠外周血PLT均升至照前值的85%,对照组为60%(P﹤0.05)。照射后14和28 d骨髓有核细胞集落培养结果提示rmIL-12治疗组CFU-GEMM均明显高于对照组(P﹤0.05)。结论 rmIL-12可明显促进急性放射病小鼠造血功能恢复。Objective To study the effect of recombinant murine interleukin 12（rmIL-12） on the hematopoietic systems in mice of acute radiation sickness.Methods Forty-two BALB/C mice were given 6.0Gy 60Co γ rays total body irradiation and randomly assigned into irradiation control group,5 and 20 μg/kg rmIL-12 treatment groups.Solvent and 5,20 μg/kg of rmIL-12 were administrated intraperitoneally 1 h following irradiation,and was administrated every 3 days after irradiation.The general conditions of mice were observed twice a day,the changes in body weight,peripheral blood cell counts were examined every three days,histopathological sections of femur were prepared to observe the histomorphological changes,and bone marrow cells were collected to perform colony cultivation on day 14 and 28 after irradiation.Results The general conditions of mice in rmIL-12 treatment group were better than those of irradiation control group.Compared with the irradiation control group,rmIL-12 5,20 μg/kg treatment significantly promoted platelet recovery,resulting in less profound nadirs（15.9% vs 8.1%,15.1% vs 8.1%,P﹤0.05） and rapid recovery to normal levels（11 days vs 14 days）.Semi-solid bone marrow cell culture also demonstrated that rmIL-12 could stimulate bone marrow cells to form more CFU-GEMM than those of the irradiation group in vitro.Conclusion RmIL-12 can significantly accelerate the recovery of hematopoietic function in acute radiation sickness mice.

关 键 词：白细胞介素12 急性放射病 造血损伤 

分 类 号：R818.71[医药卫生—放射医学]