不同利用方式下羊草草原土壤生态系统微生物群落结构的PLFA分析  

Phospholipid fatty acid analysis of microbial community structure under different land use patterns in soil ecosystems of Leymus chinensis steppes

作　　者：邹雨坤[1,2] 张静妮[1] 杨殿林[1] 陈秀蓉[2] 张天瑞[1,2] 赵建宁[1] 赵帅[1] 

机构地区：[1]农业部环境保护科研监测所,天津300191 [2]甘肃农业大学草业学院草业生态系统教育部重点实验室中-美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草业学报》2011年第4期27-33,共7页Acta Prataculturae Sinica

基　　金：国家自然科学基金(3077036731000242)资助

摘　　要：本研究利用磷脂脂肪酸(PLFA)法分析了不同利用方式对羊草草原土壤生态系统微生物群落结构的影响。结果表明,不同利用方式下羊草草原土壤微生物群落结构发生显著变化。刈割下表征土壤微生物的PLFA总量显著高于围封和放牧利用,围封条件下PLFA总量最低,相关分析表明,PLFA总量与土壤微生物量碳(熏蒸提取法)极显著正相关。刈割显著提高了土壤细菌含量,围封使土壤中放线菌含量显著增加,土壤中真菌含量在3种利用方式下则无显著变化。刈割样地表征细菌的PLFA量最高,为29.6 nmol/g,放牧和围封样地中细菌的含量分别比刈割样地降低了27.8%和49.3%。刈割样地的优势PLFA类型为a15:0,15:0,16:0和18:0,占PLFA总量的64.6%,放牧样地中优势PLFA类型为i15:0,i16:0和18:1ω9t,占PLFA总量的45.6%;围封样地中,优势PLFA类型为a15:0,15:0和i16:0,占PLFA总量的46.3%。Effects of different land use patterns on the structure of microbial communities of the Leymus chinensis steppe soil ecosystems were studied by phospholipid fatty acid analysis.Soil microbial community structure changed under different land use patterns.Microbial characterization of the total PLFA content of mown plots was significantly higher than those of the fenced and grazed plots,and total PLFA content of the fenced plots was the lowest.Correlation analysis showed that the total concentration of PLFA was significantly positively correlated with microbial biomass carbon as determined by the fumigation-extraction method.Mowing significantly increased both soil bacterial content and actinomycete content of fenced plots but soil fungal content was not significantly affected under different land use patterns.Bacterial characterization of PLFA in mown plots was the highest（29.6 nmol/g,bacterial contents） with grazed and fenced plots at 27.8% and 49.3% respectively.Dominant PLFA type of mown plots were a15：0,15：0,16：0 and 18：0,and accounted for 64.6% of total PLFA;Dominant PLFA type of grazed plots were i15：0,i16：0 and 18：1ω9t,and accounted for 45.6% of total PLFA;Dominant PLFA type of fenced plots were a15：0,15：0 and i16：0,and accounted for 46.3% of total PLFA.

关 键 词：羊草草原 微生物量碳 磷脂脂肪酸 

分 类 号：S541.9[农业科学—作物学]