分析9年不动杆菌院内感染的耐药性变化及其与I类整合子的相关性  

Analysis on variation of drug-resistance during 9 years and relation between drug-resistance and integron I of Acinetobacter

作　　者：单斌[1] 马晓波[1] 涂亮[1] 杜艳[1] 陈端[1] 

机构地区：[1]昆明医学院第一附属医院检验科,云南省650032

出　　处：《实用检验医师杂志》2011年第1期5-8,共4页Chinese Journal of Clinical Pathologist

摘　　要：目的了解我院9年间鲍曼不动杆菌和洛菲不动杆菌的耐药性变化，并探讨其耐药性与I类整合子的相关性。方法收集2001年1月至2009年12月我院分离培养的不动杆菌共1552株，采用VITEK-2全自动细菌鉴定仪进行检验及耐药分析。采用PCR检测60株鲍曼不动杆菌和30株洛菲不动杆菌的I类整合子，分析I类整合子与细菌耐药性的相关性。结果1552株不动杆菌中以鲍曼不动杆菌1360株（87．6％），洛菲不动杆菌154株（9．9％）为主。药敏结果显示不动杆菌的耐药率总体呈逐年上升的趋势。PCR检测结果显示38株（63-3％）鲍曼不动杆菌和17株（56．7％）洛菲不动杆菌为I类整合子阳性，I类整合子阳性菌株的耐药率及多重耐药（mulfidrug resistance，MDR）率显著高于I类整合子阴性菌株，且差异均有统计学意义（P均〈0．05）。结论经验性用药或抗生素滥用导致了9年间不动杆菌的耐药率呈逐年上升的趋势。I类整合子在不动杆菌的耐药率及MDR率升高中起重要作用。Objective To detect situations of drug-resistance ofAcinetobacter Baumanii andAcineto- bacter lwoffi in our hospital during 9 years, and discuss the relation between their drug-resistance and integron I. Methods 1552 strains Acinetobacter were collected and identified from January 2001 to December 2009 in our hospital. The drug sensitivity were detected by VITEK-2, and the integron I of 60 strains Acinetobacter baumanii and 30 strains Acinetobacter lwoffi were detected by PCR. The correlation between drug-resistance and integron I were analyzed. Results 1552 strains Acinetobacter main including 1360 strains （87.6%） A cinetobacter baumanii and 154 strains（9.9%） A cinetobacter lwoffi. The drug-resistance of A cinetobacter were raised year by year generally. The results of PCR showed that 38 strains（63.3%） Acinetobacter baumanii and 17 strains （56.7%） A cinetobacter lwoffi contained integron I. The drug resistance and muhidrug-resistance（MDR） of integron I positive strains were higher than integron I negative strains and there were all had statistical significance （Pall〈 0.05）. Conclusion The irrational use of antibiotics lead to the raising of A cinetobacter ＇s drugresistance rate year by year during 9 years. Integron I plays an important role in the raising of drug-resistance and MDR of Acinetobacter.

关 键 词：鲍曼不动杆菌 洛菲不动杆菌 I类整合子 多重耐药 

分 类 号：R978.1[医药卫生—药品]