显微缝合联合高脂饮食建立兔颈动脉粥样硬化模型  

Establishment and evaluation of a rabbit model of carotid artery stenosis by microsurgical suture

作　　者：王捷[1] 徐忠信[1] 李淼[1] 邵延坤[1] 王晓彬[1] 郑昭时[1] 杨宏[1] 

机构地区：[1]吉林大学中日联谊医院,吉林长春130033

出　　处：《中国实验诊断学》2011年第8期1236-1239,共4页Chinese Journal of Laboratory Diagnosis

基　　金：国家自然科学基金资助项目(30772234);吉林省自然科学基金资助项目(201015179);吉林省科技引导项目资助(20050407-2);北京市自然科学基金资助项目(7072016);北京市优秀人才培养资助个人项目(20061D0300400072);首都医科大学基础临床合作课题资助项目(2007JL39);北京市卫生系统高层次卫生技术人才培养计划(2009-3-22)

摘　　要：目的建立一种兔颈动脉粥样硬化模型并对该模型进行评价。方法应用显微缝合法(microsurgical suture)造成兔颈动脉狭窄,在此基础上联合高胆固醇饮食建立兔颈动脉粥样硬化模型,采用HE、MASSON染色切片观察不同时间颈动脉壁的病理改变,以内膜/中膜厚度比值(I/M)及内膜/中膜面积比值(SI/SM)评价动脉粥样硬化程度。结果应用显微缝合联合高脂饮食法成功建立兔颈动脉粥样硬化模型,HE、MASSON染色切片显示模型动脉内膜增厚、动脉粥样硬化斑块形成。结论应用显微缝合联合高脂饮食制作兔颈动脉粥样硬化模型,方法简便,成模时间短,极大程度保留了动脉壁的弹性和血管内皮细胞的完整性,是一种较为理想的新型动脉粥样硬化动物模型。Objective To establish and evaluate a rabbit model of carotid artery atherosclerosis.Methods Successive microsurgical suture was performed on carotid artery in rabbit in order to make angiostenosis models followed by high fat diet.Results Carotid artery atherosclerosis model performed by microsurgical suture and high fat diet successfully induced artery atherosclerosis featuers such as intima hyperplasy,lipochondria deposition as well as plaques,which were shown by HE/MASSON staining sections.Conclusion The model was an ideal animal model for atherosclerosis studying and hemodynamic analysis.The method microsurgical suture combined with high fat diet keep integrity of endothelial cells as it is possible.Furthermore,the wall of angiostenosis models keep its elastic and pulsant features.The model is a new animal model applied in study of endothelial cells＇ function and hemodynamic evaluation in AS and stenosed artery.

关 键 词：动脉粥样硬化 动物模型 显微缝合 

分 类 号：R543.4[医药卫生—心血管疾病] R-332[医药卫生—内科学]