实时定量PCR检测低剂量X射线对小鼠睾丸中CHOP和caspase-12 mRNA表达的影响  

Effects of low dose X-rays on expressions of caspase-12 and CHOP mRNAin mouse testis cells by real time quantitative PCR

作　　者：姜铁超[1] 韩宏志[2,3] 孔宁[4] 

机构地区：[1]吉林大学中日联谊医院心血管内科,吉林长春130033 [2]吉林大学公共卫生学院流行病与卫生统计学教研室吉林大学基因组医学研究中心,吉林长春130033 [3]吉林大学学报(医学版)编辑部 [4]药学院医用生物材料教研室,吉林长春130033

出　　处：《中国实验诊断学》2011年第8期1279-1281,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的通过检测低剂量X射线照射后小鼠睾丸中CHOP和caspase-12 mRNA表达的变化,在mRNA水平探讨内质网通路参与凋亡调控的可能。方法健康雄性昆明小鼠经过低剂量(0、0.050、0.075、0.100和0.200 Gy)照射后12 h和0.075 Gy照射后不同时间(0、3、6、12和24 h),应用实时定量PCR(real time quantitative PCR)检测小鼠睾丸中C/EBP同源蛋白(C/EBP homologous protein,CHOP)和胱天蛋白酶-12(caspase-12)mRNA表达剂量与时程效应关系。结果 0-0.200 Gy X射线照射后12 h,小鼠睾丸中CHOP mRNA表达随剂量逐渐增高并一直维持较高水平,其中0.050和0.100 Gy组较0 Gy显著增高(P<0.05);caspase-12 mRNA表达随剂量逐渐增高,0.075和0.100 Gy组较0 Gy组显著增高(P<0.05,P<0.01)。0.075 Gy照射后0-24 h CHOP mRNA表达随时间延长逐渐增高,在24 h到达峰值,较0 h显著增高(P<0.05);caspase-12 mRNA表达在3和6 h稍有降低后,12 h到达峰值,较0 h显著增高(P<0.05),24 h时回降。结论低剂量X线照射诱导小鼠睾丸CHOP和caspase-12 mRNA表达具有剂量-时程-效应规律性,二者可能参与低剂量X线照射诱导小鼠睾丸生精细胞凋亡的调控。Objective To detect the changes of CHOP and caspase-12 mRNA in mouse testis after low dose X rays and to investigate the possibility that endoplamic reticulum pathway regulate cell apoptosis at mRNA level.Methods Healthy male Kun Ming mouse testes 12 h after 0,0.050,0.075,0.100 and 0.200 Gy X rays irradiation and 0-24 h after 0.075 Gy X rays irradiation were got,the relationship of dose-and time-effect of CHOP and caspase-12 mRNA were detected with real time quantitative PCR.Results After 0-0.200 Gy X rays irradiation,CHOP mRNA expression in mouse testis increased with dose increasing and kept high level,it increased significantly after irradiaion with 0.050 and 0.100 Gy X rays（P0.05）;caspase-12 mRNA expression increased with dose increasing,it increased significantly after irradiaion with 0.075 and 0.100 Gy X rays（P 0.05,P0.01）.After 0.075 Gy X rays,CHOP mRNA expression increased with time prologation from 0 h to 24 h,and it reached the peak value at 24 h,it increased obviously compared with 0 h（P 0.05）;caspase-12 mRNA decreased at 3 and 6 h,but it increased at 12 h,and reached the peak value,it increased obviously compared with 0 h（P 0.05）,and it decreased again at 24 h.Conclusion Dose-and time-effect exist on the low-dose irradiation induced expressions of CHOP and caspase-12 mRNA in mouse testis,both may participate regulating spermatogenic cell apoptosis induced by low dose radiation.

关 键 词：X射线 睾丸 胱天蛋白酶12 C/EBP同源蛋白 

分 类 号：R346[医药卫生—基础医学]