双歧杆菌对过敏性哮喘儿童DC成熟及其分泌细胞因子的影响  

The effect of bifidobacterium on the maturation and the secretion of cytokine in dendritic cells from the children with allergic asthma

作　　者：马红玲[1] 王和平[1] 祖莹[1] 王莉[1] 谭卫群[1] 郑跃杰[1] 

机构地区：[1]深圳市儿童医院呼吸科,广东深圳518026

出　　处：《中国实验诊断学》2011年第8期1311-1315,共5页Chinese Journal of Laboratory Diagnosis

摘　　要：目的研究青春型双歧杆菌对过敏性哮喘儿童外周血单个核细胞(PBMC)来源的树突状细胞(DC)表达CD86和HLA-DR及其分泌IL-1βI、L-6I、L-10、IL-12、IL-23和IFN-γ的影响。方法从15名过敏性哮喘儿童和15名非哮喘儿童的外周血单个核细胞诱导生成未成熟DC,分别加入青春型双歧杆菌或脂多糖(LPS)后继续培养DC 2天,①每天于倒置显微镜下观察DC形态;②用流式细胞仪检测各组DC表面CD86和HLA-DR的分子表达;③用ELISA方法检测培养上清中IL-1βI、L-6I、L-10、IL-12I、L-23和IFN-γ的水平。结果①DC经双歧杆菌和LPS分组处理后第2天,于倒置显微镜下观察到双歧杆菌组和LPS组的DC突起均较空白组明显,具有突起的细胞数量较空白组增多,而双歧杆菌组与LPS组比较,LPS组有突起的细胞数量明显增多,提示DC过度生长。②双歧杆菌刺激后,哮喘儿童DC表面CD86表达明显增高,HLA-DR表达无明显变化,非哮喘儿童CD86和HLA-DR表达均无明显变化;LPS刺激可明显增加哮喘儿童和非哮喘儿童CD86和HLA-DR的表达。③双歧杆菌刺激哮喘儿童DC分泌IL-12、IFN-γI、L-1β及IL-6水平增高,刺激非哮喘儿童DC分泌IL-12I、L-10I、L-1β及IL-23水平增高。结论①过敏性哮喘儿童DC表面CD86的表达可能存在缺陷,双歧杆菌能适度上调其表达,在DC的成熟过程中可能起调节作用。②双歧杆菌能刺激过敏性哮喘儿童DC分泌IL-12和IFN-γ,从而改变Th2优势分化,纠正Th1/Th2失衡。③过敏性哮喘儿童DC分泌IL-10可能存在缺陷,从而影响免疫耐受的形成。④双歧杆菌可能通过刺激哮喘儿童DC分泌IL-1β及IL-6增高,达到促进Th17细胞分化的作用。Objective ①To study the effects of bifidobacterium on the expression of CD86 and HLA-DR、secretion of IL-1β、IL-6、IL-10、 IL-12、 IL-23 and IFN-γin dendritic cells（DCs） derived from peripheral blood mononuclear cells（PBMC） of the children with allergic asthma.②investigate the effect of bifidobacterium on in dendritic cells（DC） derived from the allergic asthma children peripheral blood monocytes（PBMC）.Methods The immature DCs were induced from the PBMC derived from 15 allergic asthma and 15 non-asthma children,then the immature DCs were cocultured with the bifidobacterium or LPS respectively.①Observe the shape of DC with the microscope everyday;②The expression of CD86 and HLA-DR of DCs were measured by flow cytometer;③IL-1β、IL-6、IL-10、 IL-12、 IL-23 and IFN-γ secreted by DC were measured by ELISA.Results ①The second day,The ecphymas of DCs control by LPS or Bifidobacterium is obviously more than blank observed with the microscope,And the quantity of cells with ecphymas is more than the blank too,However,The quantity of cells with ecphymas control by LPS is obviously more than that control by Bifidobacterium,From the above-mentioned,we can judge that the DCs control by LPS is overgrowth.②After the pretreatment with Bifidobacterium adolescent,the expression of CD86 of DCs from the allergic patients was significantly increased,but the expression of HLA-DR was not changed.The pretreatment with Bifidobacterium adolescent have no effects on the expression of CD86 and HLA-DR on the non-asthma children.The pretreatment with LPS may increase the expression of CD86 and HLA-DR from both the allergic patients and non-asthma children significantly.③The pretreatment with the Bifidobacterium adolescent may increase significantly the level of IL-12、IFN-γ、IL-1βand IL-6 in the patients with allergic asthma,and the level of IL-12、IL-10、IL-1βand IL-23 in the non-asthma children.Conclusion ①Bifidobacterium adolescent could stimulate the mature of DCs.The expression of CD86

关 键 词：树突状细胞 哮喘 双歧杆菌 CD86 HLA-DR IL-1β L-6 L-10 IL-12 IL-23 FN-γ 

分 类 号：R725.6[医药卫生—儿科]