肝郁-心理应激对子宫肌瘤大鼠性激素及免疫因子影响的实验研究  

Experimental Study of Effect of Liver Qi Stagnation-psychological Stress on Sex hormone and Immune Factors in Uterine Leiomyomas Animal Model

作　　者：朱静 夏天[2] 刘兆娟[3] 

机构地区：[1]金坛中医医院,江苏金坛213200 [2]天津中医药大学第一附属医院,天津300193 [3]天津南开医院,天津300193

出　　处：《辽宁中医药大学学报》2011年第9期52-56,共5页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：国家自然基金面上项目(81072838)

摘　　要：目的:探讨肝郁-心理应激在ULM发病机制中的重要作用,为临床从肝郁-心理应激角度防治ULM提供理论依据,并为中医学"情志学说"提供新的理论依据。方法:将80只SD雌性大鼠按完全随机设计分为正ULM组大鼠建立慢性束缚应激模型,ULM模型组及肝郁+ULM组大鼠建立子宫肌瘤模型,16周后处死全部大鼠,IL-6、INF-γ、TNF-α等细胞因子的含量。结果:肝郁-心理应激可明显增加大鼠子宫重量及系数(P<0.05或P<0.01),降低血清E2、P含量(P<0.05或P<0.01),升高血清LH、FSH含量(P<0.05或P<0.01),并可使胸腺指数明显降低(P<0.01),降低血清IL-2、INF-γ含量(P<0.05或P<0.01),升高血清IL-6、TNF-α含量(P<0.05)。结论:肝观察各组大鼠一般行为学改变,测量子宫、胸腺、脾脏湿重,并计算各脏器指数,HE染色后观测各组大鼠子宫病理组织学变化,双抗体夹心酶联免疫吸附技术(ELISA)测定大鼠血清中E2、P、FSH、LH等性激素含量及血清中IL-2、Objective：Discuss the role of Liver qi stagnation-psychological which was played in the pathogenesis of uterine leiomyomas,provide the oretical basis of clinical therapy of uterine leiomyomas from the liver qi stagnation-psychological stress view and a new theory in Chinese medicine＂emotions theory＂.Methods：A total of 80 Kunming mice were randomized into normal control group,ULM group,liver qi stagnation-psychological stress group,liver qi stagnation-psychological stress-uterine leiomyomas group;each group contained 20 mice.Chronic unpredictable mild stress models were as tablished in liver qi stagnation-psychological stress group and liver qi stagnation-psychological stress-uterine leiomyomas group;uterine leiomyomas models were as abolished in ULM group and liver qi stagnation-psychological stress-uterine leiomyomas group.After 16 weeks,The weight of uterine,thymus index,spleen index were measured;Uterine pathology were detected after HE;The level of E2,P,LH,FSH in serum were detected by enzyme-linked immunoabsorbent assay（ELISA）;The level of IL-2,IL-6,INF-γ,TNF-α in serum were Detected by enzyme-linked immunoabsorbent assay（ELISA）.Results：Liver qi stagnation-psychological stress significantly increased the weight and coefficient of uterus in rats（P〈0.05 or P〈0.01）,inclined the contents of E2,P in serum（P〈0.05 or P〈0.01）,increased the contents of LH,FSH in serum of rats（P〈0.05 or P〈0.01）,and significantly inclined the thymus index（P〈0.01）,inclined the contents of IL-2,INF-γ in serum（P〈0.05 or P〈0.01）,increased the contents of IL-6,TNF-α in serum（P〈0.05）.Clonclusion：Liver qi stagnation-psychological stress can lead to endocrine dysfunction,impair the immune function and promoting the grouth of uterine fibroid.

关 键 词：大鼠子宫肌瘤 肝郁-心理应激 性激素 免疫因子 

分 类 号：R322.47[医药卫生—人体解剖和组织胚胎学]