肾消康对糖尿病大鼠肾组织基因myosin Va表达的影响  

Effect of Shenxiaokang on Diabetic Nephropathy Rats Renal Tissues Gene-myosin Va Expresstion

作　　者：白玉宾[1] 王兵[1] 刘春红[1] 柳成刚[1] 郭加利[1] 宁式颖[1] 韩洁茹[1] 解颖[1] 陈飞[1] 姜德友[1] 

机构地区：[1]黑龙江中医药大学,黑龙江哈尔滨150040

出　　处：《辽宁中医药大学学报》2011年第9期57-59,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：黑龙江省杰出青年科学基金课题(2070222)

摘　　要：目的:观察中药复方肾消康对糖尿病肾病大鼠的防治作用及对肾脏基因表达的影响,探讨糖尿病肾病(DN)的发病机制,揭示中药复方肾消康防治DN的作用机理,筛选药效作用靶点,为临床推广应用提供科学依据。方法:本实验采用链脲佐菌素(STZ)加高热量饮食建立糖尿病大鼠肾脏损伤模型。运用放免、生化、光镜、电镜及美国Affymetrix公司的基因表达谱芯片、RT-PCR等先进检测手段和方法,观察了多项指标,尤其是通过聚类分析寻找和DN有重要相关性的基因,并进一步采用实时荧光定量RT-PCR法做验证实验研究。结果:肌球蛋白Va(myosin Va),在糖尿病大鼠肾脏表达下调,肾消康治疗组表达上调。结论:应用Affymetrix Rat2302.0基因表达谱芯片检测糖尿病大鼠肾脏基因的表达,myosin Va是筛选出的肾脏差异表达基因和药效靶点之一,并对该基因做荧光定量RT-PCR测序分析,这在国内外尚属首次。基因功能分析表明,在糖尿病状态下,myosin Va表达下调,与糖尿病肾脏损伤有重要相关性,而经肾消康治疗后大鼠肾脏组织中myosin Va表达上调,可见肾消康对myosin Va基因表达具有良性调节作用,其作用机制还有待于进一步研究。Objective：To observe the therapeutically effect of a complex prescription of Shenxiaokang on DN rats and discuss the mechanism of action in the treatment of DN,then provide experimental data for clinical practice.Methods：The model rats male Wister was established with multiple injections of low dose STZ and high calorie diet.Kinds of examination methods were applied,including radioimmunoassay,biochemistry,light microscope,electron microscope,immunohistochemical,Affymetrix Co.US.Rat 2302.0 gene expression profiling chip,and real time quantitative reverse transcription polymerase chain reaction technique to observe the effect of Shenxiaokang on indicators.Most especially applied cluster analysis method to make certain important associated genes with DN,and further applied RT-PCR to do confirmatory experiment.Results：the myosin VA gene was down-regulation in the DN model group rat kidneys and up-regulation in the Shenxiaokang treatment group rat kidneys.Conclusions：Applied Affymetrix Co.US.Rat 2302.0 gene expression profiling chip to test Diabetic nephropathy Rats renal tissues Genes expression.Myosin Va gene was one of the renal tissues difference Expresstion and drug effect targets.And Applied real time quantitative reverse transcription polymerase chain reaction technique to analyze it.It was the first time at home and abroad.To genes function analyze show that,the myosin Va gene was down-regulation in the DN model group rat kidneys.It was important associated with Diabetic kidney damage,and up-regulation in the Shenxiaokang treatment group rat kidneys,Shenxiaokang had benign effect on myosin Va gene Expresstion.Its mechanism remains to be studied further.

关 键 词：肾消康 糖尿病 肾脏损伤 基因表达谱 肌球蛋白Va 

分 类 号：R587.1[医药卫生—内分泌]