RP-HPLC法测定排石利胆颗粒中芍药苷含量  

Determination of Paeoniflorin in Paishi Lidan Granule by RP-HPLC

作　　者：张颖[1] 张起辉[2] 邸子真[1] 邹桂欣[1] 尤献民[1] 

机构地区：[1]辽宁省中医药研究院,辽宁沈阳110034 [2]重庆大学化学化工学院,重庆400030

出　　处：《辽宁中医药大学学报》2011年第9期197-198,共2页Journal of Liaoning University of Traditional Chinese Medicine

摘　　要：目的:建立排石利胆颗粒中芍药苷的含量测定方法。方法:采用RP-HPLC法测定含量,使用Hypersil C18(5μm,4.6mm×200mm)色谱柱,紫外检测波长230nm,乙腈-0.1%磷酸(13:87)为流动相,流速1.0mL/min。结果:通过方法学考察,芍药苷在0.08104～0.48624μg与峰面积呈良好的线性关系,r=0.9996,平均回收率96.2%(RSD=1.1%,n=9)。结论:RP-HPLC法可用于排石利胆颗粒中芍药苷的含量测定。Objective：To establish the determination method of the concent of paeoniflorin in Paishi Lidan granule by HPLC.Method：The column was Hpersil C18（5μm,4.6mm×200mm）,the detection wavelength was at 230nm,the mobile phase was the mixture of acetonitrile-0.1% phosphoric acid（13：87）,the flow rate is 1.0mL/min.Result：A good linear correlation of paeoniflorin was observed between 0.08104～0.48624μg,r =0.9996,the average recovery was 96.2% with RSD of 1.1%（n=9）.Conclusion：The method can be used as the quality control method of this preparation.

关 键 词：RP—HPLC 排石利胆颗粒 芍药苷 

分 类 号：R284.1[医药卫生—中药学]