泌尿生殖道沙眼衣原体临床株体外药物敏感性检测及分析  

The Detection and the Susceptibility Analysis to Antimicrobial Agants of Urogenital Chlamydial Trachomatis Infection

作　　者：吴志周[1] 祝新[1] 杨日好[1] 柯建良[1] 黄澍杰[1] 

机构地区：[1]广东省江门市皮肤医院,529000

出　　处：《医学理论与实践》2011年第15期1777-1778,1786,共3页The Journal of Medical Theory and Practice

摘　　要：目的：检测江门地区泌尿生殖道沙眼衣原体临床分离株对强力霉素、四环素和左氧氟沙星等8种临床常用抗生素的体外药物敏感性。方法：McCoy细胞培养法分离沙眼衣原体临床株,测定8种抗生素对沙眼衣原体的最低抑菌浓度（MIC）。结果：共培养出沙眼衣原体阳性株100株,经传代培养至感染率达90%以上符合药敏试验所需菌量的菌株有73株;MIC值范围分别为强力霉素0.016~0.125μg/ml、四环素0.039~0.625μg/ml、阿奇霉素0.032~1μg/ml、交沙霉素0.005~0.16μg/ml、红霉素0.125~2μg/ml、克拉霉素0.004~0.064μg/ml、左氧氟沙星0.063~1.0μg/ml和司帕沙星0.032~0.256μg/ml。结论：克拉霉素及阿奇霉素有较强的抗菌活性,强力霉素次之,红霉素和左氧氟沙星的抗菌活性则较低。Objective：To assess the vitro susceptibility to 8 antimicrobial agents of clinical isolates of Chlamydia trachomatis（Ct） from Jiangmen region.Methods：Ct was isolated from clinical specimens by using McCoy cell culture and subjected to propagation.The minimum inhibitory concentration of 8 antimicrobial agents（Doxycycline,Tetracycline,Azithromycin,Josamycin,Erythrocin,Clarithromycin,Levofloxacin and Sparfloxacin）against Ct isolates were determined.Results：A total of 100 clinical strains were isolated from cervical or urethral swab samples and propagated in McCoy cells,and 73 strains that the infection rate reached more than 90% met the requirements for susceptibility testing after serial propagation.The MICs rang of Doxycycline,Tetracycline,Azithromycin,Josamycin,Erythrocin,Clarithromycin,Levofloxacin and Sparfloxacin were as follows：0.016~0.125μg/ml,0.039~0.625μg/ml,0.032~1μg/ml,0.005~0.16μg/ml,0.125~2μg/ml,0.004~0.064μg/ml,0.063~1.0μg/ml and 0.032~0.256μg/ml.Conclusion：Clarithromycin and Azithromycinand exhibit an excellent activity against Ct.Doxycycline,Erythromycin and Levofloxacin followed with a lower antimicrobial activity.

关 键 词：沙眼衣原体 药物敏感性 最低抑菌浓度 

分 类 号：R446.5[医药卫生—诊断学]