不同取代度N-邻苯二甲酰壳聚糖抗氧化活性的研究  

Antioxidant Activity of N-Phthaloyl-Chitosan Oligosaccharide with Different Substituting Degrees

作　　者：孙涛[1] 银旭红[1] 谢晶[1] 康永锋[1] 周冬香[1] 

机构地区：[1]上海海洋大学食品学院,上海201306

出　　处：《天然产物研究与开发》2011年第4期713-716,738,共5页Natural Product Research and Development

基　　金：上海市教委重点学科建设项目(J50704);上海市重点学科建设项目专项基金(T1102);上海市生物医药和农业科技领域重点科技项目(08391911500);2009年上海市优秀学科带头人计划(09XD1402000)

摘　　要：低聚壳聚糖与邻苯二甲酸酐酰化得到三种取代度不同的N-邻苯二甲酸酐酰低聚壳聚糖NPCOSA、NPCOSB和NPCOSC,取代度分别为0.330、.55和0.65。通过红外光谱对其结构进行表征。并考察了其抗氧化性能。结果表明:COS的抗氧化性能最强;随着取代度的增加,N-邻苯二甲酰低聚壳聚糖对超氧阴离子的清除能力逐渐升高;而对DPPH的清除能力以及还原能力呈逐渐下降趋势;对羟基自由基的清除顺序大小依次为NPCOSB>NPCOSA>NPCOSC,即NPCOSB清除羟基自由基的的能力最佳。N-phthaloyl chitosan oligosaccharides（NPCOSA,NPCOSB and NPCOSC）,having various degrees of substitution（DS were 0.33,0.55 and 0.65,respectively）were prepared by chemical modification of chitosan oligosaccharide.The chemical structures of the chitosan oligosaccharides derivatives were characterized by FTIR.Their antioxidant activities were evaluated by the scavenging of superoxide anion radicals（O-·2）,hydroxyl radical（·OH）,DPPH radical and deter mination of reducing power.The results indicated that all the N-phthaloyl chitosan oligosaccharides showed lower antioxidant activity than chitosan oligosaccharides,the scavenging effect on O-·2 increased with the increasing of DS,and their scavenging effect on DPPH and reducing power both were decreased with the increasing of DS;the order of their scavenging effect on ·OH was NPCOSBNPCOSANPCOSC,and the NPCOSB showed the strongest scavenging effect on ·OH.

关 键 词：低聚壳聚糖 N邻苯二甲酰化低聚壳聚糖 抗氧化性 

分 类 号：TS202.3[轻工技术与工程—食品科学]