二噁英诱导胎鼠腭裂组织中TGF-β3表达变化  

Expression of TGF-β3 in mice with 2,3,7,8-tetrachlorodibenzo-p-dioxin-induced palatal cleft

作　　者：蒲亚兰[1] 刘丽玲[1] 甘立强[1] 傅跃先[1] 

机构地区：[1]重庆医科大学儿童发育疾病研究省部共建教育部重点实验室,重庆400014

出　　处：《中国公共卫生》2011年第9期1203-1205,共3页Chinese Journal of Public Health

基　　金：重庆市自然科学基金重点项目(2009BA5085)

摘　　要：目的探讨转化生长因子(TGF-β3)在2,3,7,8-四氯二噁英(TCDD)诱导C 57 BL/6 J胎鼠腭裂发生中的作用。方法在小鼠怀孕第10 d(GD 10),二噁英组以TCDD 64μg/kg灌胃,对照组以玉米油16 mL/kg灌胃,于GD18.5解剖显微镜下观察胎鼠腭裂发生率,并于GD 13.5、14.5、15.5剪取胎鼠腭突组织提取RNA及蛋白,采用逆转录聚合酶链反应(RT-PCR)和western blot分别检测腭突组织中TGF-β3 mRNA和TGF-β3蛋白表达情况。结果二噁英组腭裂发生率为100%(39/39),对照组无腭裂发生(0/42);在GD 13.5、14.5、15.5时,二噁英组TGF-β3 mRNA相对表达量分别为(0.392±0.056)、(0.467±0.064)、(0.192±0.032),明显高于对照组(0.081±0.020)、(0.178±0.011)、(0.113±0.016)(P<0.05);二噁英组TGF-β3蛋白相对表达量分别为(0.046±0.009)、(0.089±0.015)、(0.028±0.003),明显高于对照组(0.017±0.001)、(0.039±0.003)、(0.005±0.001)(P<0.05)。结论 TCDD可诱导C 57 BL/6 J胎鼠形成腭裂,并明显上调胎鼠腭突组织中TGF-β3表达。Objective To explore the effect of transforming growth factor-β3 （TGF-β3） on palatal cleft in C57BL/6J mice induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin（TCDD）. Methods On gestation day 10（GD 10） ,the pregnant mice were randomly divided into two groups：the treated group（ with TCDD exposure at the dose of 64 μg/kg body weight） and the control group（with corn oil of 16 ml/kg body weight）. The embryos were examined under stereomicroscope to detect the incidence of deft palate on GD 18.5 and the palatal shelves were dissected from the embryos for RNA and protein extractions on GD 13.5 ,GD 14. 5 ,and GD 15.5. The expression of TGF-β3 mRNA and protein were detected with reverse transcription-PCR（RT-PCR） and western blot. Results The incidence of cleft palate was 100% in TCDD group（39/39） and there was no cleft palate observed in the control group（0/42）. The expression of TGF-β3 mRNA and protein were grad- ually up-regulated in TCDD group on different phases of the development of palatal shelves （ P 〈 0. 05 ）. Conclusion TCDD can induce a stable formation of cleft palate and the up-regulation of the TGF-β3 expression and the over expression of TGF-β3 may be a key signal pathway resulting in TCDD-induced cleft palate.

关 键 词：腭裂 2 3 7 8-四氯二噁英 转化生长因子Β3 

分 类 号：R114[医药卫生—卫生毒理学]