银掺杂聚L-缬氨酸修饰电极的制备及对肾上腺素的测定  

Preparation of a silver doped poly(L-Valine) modified electrode and Its determination for epinephrine

作　　者：孙登明[1] 胡文娜[1] 马伟[1] 

机构地区：[1]淮北师范大学化学与材料科学学院,淮北235000

出　　处：《分析试验室》2011年第9期50-54,共5页Chinese Journal of Analysis Laboratory

基　　金：安徽省科技计划重点基金项目(07020203011);安徽省高校省级自然科学研究重点基金项目(KJ2008A122;KJ2011A255)资助

摘　　要：利用循环伏安法将银和L-缬氨酸聚合修饰在玻碳电极表面,制成银掺杂聚L-缬氨酸修饰电极(Ag-PLV/GCE),研究了肾上腺素在该电极上的电化学行为,建立了电化学测定肾上腺素的新方法。在pH3.5的磷酸盐缓冲溶液中,扫描速率为40 mV/s时,肾上腺素在修饰电极上产生一对氧化还原峰,Epa=0.422 V,Epc=0.390 V,肾上腺素的电子转移系数为0.54,表观反应速率常数为1.02/s。用线性扫描伏安法进行测定时,峰电流与肾上腺素浓度在5.0×10-8～1.0×10-5 mol/L范围内呈良好的线性关系,检出限为1.0×10-8 mol/L。In this paper,a modified electrode was developed by electrochemical immobilization of silver and L-valine on a glassy carbon electrode surface.The voltammetric behavior of epinephrine was studied at the silver doped poly（L-valine） modified electrode.In phosphate buffer solution at pH3.5,with the scan rate of 40 mV/s,a pair of redox peaks were observed with the peak potentials at Epa =0.422 V and Epc=0.390 V（υs Ag/AgCl）.The apparent electron transfer coefficient and standard rate constant of the oxidation of epinephrine were 0.54 and 1.02/s,respectively.In liner sweep voltammetry,the oxidation peak current of epinephrine was proportional to the concentration in the range of 5.0×10-8 ~ 1.0×10-5 mol/L.The detection limit was 1.0×10-8 mol/L.The method was successfully applied to for the determination of epinephrine in injections and urine samples with satisfactory results.

关 键 词：肾上腺素 银掺杂 L-缬氨酸 聚合物修饰电极 循环伏安法 线性扫描伏安法 

分 类 号：O657.1[理学—分析化学]