GC-MS法测定虎杖膏中龙脑与异龙脑的含量  

Content Determination of Borneol and Isoborneol in Huzhang Cream by GC-MS

作　　者：易学东[1] 娄方明[2] 蔡义云[2] 

机构地区：[1]遵义医学院附属医院,遵义市563000 [2]遵义医学院,遵义市563000

出　　处：《中国药房》2011年第35期3313-3314,共2页China Pharmacy

基　　金：贵州省中医药管理局中医药;民族医药科学技术研究课题(D-336)

摘　　要：目的:建立测定虎杖膏中龙脑与异龙脑含量的方法。方法:采用气相色谱-质谱联用法。色谱柱为Agilent HP-5MS(30m×0.25mm×0.25μm),程序升温,汽化室温度为260℃,载气为高纯He,流速为1mL·min-1,分流比为20:1;离子源温度为230℃,四极杆温度为150℃,电离方式为EI,进样量为2μL。结果:龙脑和异龙脑的进样量分别在0.012～0.150、0.005～0.061μg范围内与各自峰面积积分值呈良好的线性关系(r均为0.9997);二者平均加样回收率分别为99.56%(RSD=2.20%,n=6)和99.79%(RSD=2.03%,n=6)。结论:本方法稳定、简便、灵敏、快速,可用于虎杖膏中龙脑与异龙脑的含量测定。OBJECTIVE： To establish a method for the content determination of borneol and isoborneol in Huzhang cream. METHODS： GC-MS method was adopted. The Agilent HP-5MS chromatographic column was used with programm,ted temperature, high purity He as carrier gas and the vaporizer temperature at 260 ℃. The flow rate was 1 mL· min^-1, the sample size was 2 μL amd split ratio 20： 1. The ion source temperature was 230 ℃ and quadropole temperature was 150 ℃. Ionization mode was El. RESULTS： The isobomeol and borneol both showed a good linear relationship were at the range of 0.005-0.061 μg and 0.012-0.150 μg, respectively（r=0.999 7）. The average recoveries were 99.56% （RSD=2.20% ,n=6）and 99.79% （RSD=2.03% ,n=6）,respectively. CONCLUSION： The method is stable, simple, sensitive and rapid. It can be used for the content determination of isobomeol and borneol in Huzhang cream.

关 键 词：气相色谱-质谱联用法 虎杖膏 龙脑 异龙脑 含量测定 

分 类 号：R283.621[医药卫生—中药学] R927.2[医药卫生—中医学]