家蝇幼虫抗菌肽MDL-2干扰细菌功能的初步研究  

A temporary Study on antimicrobial peptide MDL-2 from Musca domestica Larvae interfering metabolism of bacteria

作　　者：宫霞[1] 乐国伟[2] 

机构地区：[1]上海商学院食品学院,上海200235 [2]江南大学食品学院,江苏无锡214036

出　　处：《河南农业大学学报》2011年第4期437-441,共5页Journal of Henan Agricultural University

基　　金：上海市教委科研创新项目(09YZ470);中央级科研院所基础性专项资金工作项目重点项目(2001DEA-20022)

摘　　要：研究了家蝇幼虫抗菌肽MDL-2对细菌细胞壁的溶解作用、细胞膜渗透性和代谢的影响.抗菌肽MDL-2在抗菌过程中首先与细菌的细胞壁相互作用,使其破裂.菌肽对革兰氏阴性细菌大肠杆菌的细胞壁的作用有浓度依赖性,而对革兰氏阳性细菌金黄色葡萄球菌,MDL-2在较低的浓度时即具有破坏细胞壁的作用;抗菌肽MDL-2与金黄色葡萄球菌的细胞膜作用曲线呈现典型的S型,有较强的协同效应,并随着作用时间的延长细胞的渗透性增强;抗菌肽对细菌的代谢功能有较强的干扰作用,阻碍菌体的生命活动,加速菌体死亡.Effects of antimicrobial peptide MDL-2 from Musca domestica Larvae on dissolution and permeability and metabolism of bacteria were studied in this paper.The cell wall of bacteria was ruptured by interaction antimicrobial peptide MDL-2 with bacteria,gram-negative bacteria,E.coli had concentration-dependency when antibacterial peptide MDL-2 assembled to certain concentration on cell outer membrane;The cell wall of gram-positive bacteria,S.aureus was fallen apart in lower concentration of MDL-2.The S-type graph showed that synergism MDL-2 interplayed with cytoplasmic of S.aureus;metabolism function and bionergy of bacteria were interfered with antibacterial peptide MDL-2,accelerating bacteria＇s death.

关 键 词：家蝇幼虫 抗菌肽 渗透性 Β-半乳糖苷酶 代谢 

分 类 号：Q966[生物学—昆虫学]