电针对局灶性脑缺血再灌注大鼠Bcl-2、Bax表达的影响  

Effects of Electro-Acupuncture on Bcl-2 and Bax of Rats with Focal Cerebral Ischemia Reperfusion

作　　者：黄浏姣[1] 洪亚群[2] 陈邦国[2] 郑慧慧[2] 

机构地区：[1]湖北中医药大学临床医学院,武汉430065 [2]湖北中医药大学针灸骨科学院,武汉430065

出　　处：《中国康复》2011年第2期83-85,共3页Chinese Journal of Rehabilitation

基　　金：教育部高等学校博士学科点专项科研基金(20094230110002)

摘　　要：目的:探讨电针对局灶性脑缺血再灌注(MCAO)模型大鼠缺血脑组织Bcl-2、Bax蛋白表达的影响。方法:选用雄性SD大鼠140只,随机分为正常组、假手术组各10只,剩余的120只大鼠采用改良Longa动脉线栓法制作MCAO大鼠模型,将造模成功的108只大鼠随机分为模型组和电针组各54只。电针组大鼠于造模后1 h开始电针百会、水沟及双侧后三里穴,30 min。正常组及假手术组10只大鼠及模型组、电针组各取9只大鼠于3、6、12、24、48及96 h各时点断头取缺血区脑组织行免疫组织化学S-P法检测Bcl-2及Bax蛋白阳性表达。结果:①正常组和假手术组大鼠脑组织中有少量Bcl-2及Bax蛋白阳性表达;与正常组及假手术组比较,模型组及电针组6、12、24、48及96 h点均阳性表达明显增多(P<0.05),于24 h达高峰,之后逐渐下降。电针组Bcl-2蛋白各时点均高于模型组,Bax明显低于模型组(P<0.05)。结论:电针对局灶性脑缺血再灌注模型大鼠脑损伤有保护作用,其作用机制可能与上调Bcl-2、下调Bax蛋白表达有关。Objective： To explore the effects of electro-acupuncture on the expression of Bcl-2 and Bax in ischemic brain tissues of rat focal cerebral ischemia reperfusion model.Methods：The arterial line bolt method improved by Longa was used to make MCAO rat model.The 140 SD rats were randomly divided into normal control group,sham-operation group,MCAO model control group and electro-acupuncture group.Each of the last two groups was divided into six subgroups（3 h,6 h,12 h,24 h,48 h and 96 h after ischemia-reperfusion）.The immunohistochemical SP method was used to observe the Bcl-2 and Bax expression levels in rat ischemic brain tissues under the light microscopy.Results：（1） In the normal control group and sham-operation group,there was weak expression of Bcl-2 in brain tissues.The Bcl-2 expression in the MCAO model group and electro-acupuncture group was increased significantly as compared with the normal control group and sham-operation group（P0.05）,which reached a peak at 24 h.In electro-acupuncture 6 h,12 h,24 h,48 h,96 h sub groups,the positive expression rate of Bcl-2 was higher than in the MCAO model group（P0.05）;（2） In the normal control group and sham-operation group,there was weak expression of Bax in brain tissues.The positive expression rate of Bax in electro-acupuncture group was significantly higher than in the MCAO model group（P0.05）,which reached a peak at 24 h.In the electro-acupuncture 6 h,12 h,24 h,48 h,96 h subgroups,the Bax positive expression rate was higher than in the MCAO model group（P0.05）.Conclusion The Electro-acupuncture therapy had a protective effect against the focal cerebral ischemia-reperfusion injury in rats probably by increasing Bcl-2 and reducing Bax levels.

关 键 词：电针 脑缺血再灌注 BCL-2 BAX 

分 类 号：R245[医药卫生—针灸推拿学]