补充丙酮酸钙对急性力竭运动小鼠心肌损伤的保护机制  

Protective Effect of Calcium Pyruvate Supplementation on Cardiac Injury after Exhaustive Exercise in Mice

作　　者：陈永亮[1] 吕国枫[2] 刘丽红[1] 张美[1] 佟梦萦[1] 孙娇[1] 方静[1] 

机构地区：[1]大连医科大学,七年制辽宁大连116044 [2]大连医科大学基础医学院,辽宁大连116044

出　　处：《中国康复》2011年第3期171-173,共3页Chinese Journal of Rehabilitation

摘　　要：目的:观察补充丙酮酸钙对小鼠心脏一氧化氮(NO)及NO合酶(NO-NOS)体系及线粒体Ca2+的影响。方法:雄性小鼠32只,随机分为安静对照组(C组)、运动对照组(Ec组)、丙酮酸钙组(P组)、运动丙酮酸钙组(Ep组)各8只。P组、Ep组小鼠每天给予600 mg/kg.d的丙酮酸钙灌胃;C组、Ec组给予同等剂量蒸馏水灌胃。3周末时Ec组和Ep组小鼠进行力竭运动实验1次后,将4组小鼠处死。检测各组心脏组织中NO、TNOS,iNOS及心脏线粒体中的Ca2+浓度。结果:与C组比较,Ec组及Ep组小鼠心脏组织TNOSi、NOS及NO、线粒体Ca2+的浓度均明显上升(P<0.05,0.01);Ep组明显低于Ec组(P<0.05);P组与C组比较,各项指标均无统计学意义。结论:补充3周的丙酮酸钙能明显降低急性运动引起的心肌NO和线粒体Ca2+浓度。为补充丙酮酸钙对心肌运动损伤的保护作用机制提供一定的实验依据。Objective： To investigate the protective effect of calcium pyruvate supplementation on cardiac NO-NOS system and mitochondria Ca2＋ after exhaustive exercise in mice.Methods： Thirty-two mice were randomly divided into four groups： control group,exercise control group,pyruvate group and exercise＋pyruvate group.The mice in pyruvate groups received calcium pyruvate,and those in control groups received placebo 600 mg/kg every day for 3 weeks.NO,TNOS,iNOS and Ca2＋ were measured after the experiment.Results： There was a significant increase in TNOS,iNOS,NO,Ca2＋（P0.05,0.01） in exercise control group as compared with control group at the end of exhaustive exercise.At the end of the experiment,there was a significant decrease in NO,TNOS,iNOS and Ca2＋（all P0.05） in exercise＋pyruvate group as compared with exercise control group.Conclusion： Supplementation of calcium pyruvate for 3 weeks can decrease NO and Ca2＋ in the heart after exhaustive exercise in mice.

关 键 词：丙酮酸钙 一氧化氮 一氧化氮合酶 钙 

分 类 号：R87[医药卫生—运动医学]