响应曲面法优化微波辅助提取胭脂虫红色素工艺  

Optimizing Conditions for Microwave-assisted Extraction of Cochineal Dye Using Response Surface Method

作　　者：郭元亨[1,2] 马李一[1,2] 郑华[1,2] 张弘[1,2] 甘瑾[1,2] 李凯[1,2] 涂行浩[1,2] 

机构地区：[1]中国林业科学研究院资源昆虫研究所 [2]国家林业局资源昆虫培育与利用重点实验室,云南昆明650224

出　　处：《林产化学与工业》2011年第4期87-92,共6页Chemistry and Industry of Forest Products

基　　金：科技部成果转化项目(2007GB24320425);国家林业科技成果推广项目([2010]11)

摘　　要：利用微波辅助水提取胭脂虫红色素,通过单因素试验考察了提取条件对色素得率的影响。结果表明,5 g胭脂虫干样,微波处理前常温浸泡40 min,液料比7∶1(g∶mL),微波功率700 W,辐射60 s后75℃水浴浸提40 min适宜于色素提取。在单因素试验的基础上,进行3因素3水平响应面分析试验,得到最优条件为5 g胭脂虫干样,液料比值7.5,微波功率714 W,辐射时间73 s,色素得率可达7.30%。最优条件下,合适的提取次数为12次,色素总得率达到29.95%,提取率为95.13%。Cochineal（Dactylopius coccus Costa） dye was extracted by microwave-assisted extraction with water.Effects of single-factor test on the yield of cochineal dye was carried out.The results show that for 5 g cochineal,the suitable conditions for extraction of dye are cochineal sample soaked in water for 40min before microwave treatment,ratio of liquid to material 7 ∶1（g ∶mL）,microwave power 700 W,microwave treatment time 60 s,and extract with water-bath at 75 ℃ for 40 min after microwave treatment.According to the single-factor test,the microwave-assisted extraction process parameters for extraction of cochineal dye were optimized using response surface method.The results show that the optimum parameters should be as follows： cochineal 5 g,ratio of liquid to material 7.5,microwave power 714 W,microwave treatment time 73 s.The extraction yield of conchineal dye is up to 7.30%.Under this conditon the reasonable extracting times is 12,the total yield of conchineal dye is 29.95 % and extraction yield is 95.13 %.

关 键 词：胭脂虫红色素 微波 提取 响应面优化 

分 类 号：TQ351[化学工程] S899.3[农业科学—特种经济动物饲养]