双黄连冻干粉对肺炎克雷伯菌抑制作用的实验研究  

Experimental Study on the Bacteriostasis Action of Freeze-dried Shuanghuanglian Powder on Klebsiella Pneumoniae

作　　者：郭丽双[1] 李凯军[1] 马微[1] 付英梅[2] 张文莉[2] 张凤民[2] 

机构地区：[1]牡丹江医学院,黑龙江牡丹江157011 [2]哈尔滨医科大学,黑龙江哈尔滨150086

出　　处：《中医学报》2011年第8期914-916,共3页Acta Chinese Medicine

基　　金：牡丹江医学院科研项目(编号:2010-03)

摘　　要：目的:探讨双黄连冻干粉对肺炎克雷伯菌的抑制作用。方法:选择SHV型产ESBLs肺炎克雷伯菌,采用微量肉汤稀释法,依据不同浊度液体内细菌含量不同的原理,绘制时间-浊度曲线(生长曲线),并对结果进行分析。结果:不同浓度双黄连作用下,肺炎克雷伯菌呈现不同的生长状态。对SHV型肺炎克雷伯菌标准株(ATCC700603)而言,当双黄连浓度<12.5 g/L时,对菌株生长无明显作用,当浓度为12.5 g/L时,对其生长有明显抑制作用;而对于SHV型产ESBLs肺炎克雷伯菌临床株,不同浓度的双黄连均显示抑制细菌生长的作用,抑制作用随着浓度的增加而增强;当双黄连浓度为50 g/L时,所有细菌均不生长。结论:双黄连冻干粉对肺炎克雷伯菌生长具有明显的抑制作用。Objective：To explore the bacteriostasis effect of freeze-dried Shuanghuanglian powder on Klebsiella pneumoniae.Methods：According to the principle that liquids of different turbidity have different bacterium concentration,SHV-type ESBLs Klebsiella pneumoniae were selected to draw up the time-turbidity curve by broth microdilution method.Results：Klebsiella pneumoniae showed different growth status under different concentrations of Shuanghuanglian.As far as the standard strain（ATCC700603） of SHV-type Klebsiella pneumoniae was concerned,freeze-dried Shuanghuanglian powder had no significant effect on the growth of bacteria when the concentration of Shuanghuanglian was below 12.5 g/L,however,the growth of the standard strain of Klebsiella pneumoniae was significantly inhibited when Shuanghuanglian concentration was at 12.5 g/L.For the clinical strains of Klebsiella pneumoniae,different concentrations of Shuanghuanglian all had bacteriostasis effect on Klebsiella pneumoniae,and the bacteriostasis effect enhanced with the increase of concentration.The growth of all kinds of bacteria was completely inhibited under the concentration of Shuanghuanglian at 50 g/L.Conclusion： Freeze-dried Shuanghuanglian powder has significant bacteriostasis effect on Klebsiella pneumoniae.

关 键 词：双黄连冻干粉 肺炎克雷伯菌 生长曲线 

分 类 号：R259.631[医药卫生—中西医结合]