1,3-环己二酮荧光法测定食品中的痕量甲醛  

Spectrofluorometric determination of trace amounts of formaldehyde in food after derivatization with 1,3-Cyclohexanedione

作　　者：张建霞[1,2] 郭惠[1] 刘希东[1] 李原芳[2] 

机构地区：[1]重庆文理学院化学与环境工程学院,重庆永川402160 [2]西南大学化学化工学院重庆市现代分析化学重点实验室,重庆北碚400715

出　　处：《重庆文理学院学报（自然科学版）》2011年第4期50-53,共4页Journal of Chongqing University of Arts and Sciences

基　　金：重庆市教委科学技术研究项目(KJ071209);重庆市现代分析化学重点实验室开放基金计划资助项目(201001)

摘　　要：甲醛与1,3-环己二酮-组胺在弱酸性缓冲介质中反应生成强荧光化合物,由此建立一种测定食品中痕量甲醛的荧光分光光度法.在优化的实验条件下,反应体系的相对荧光强度与甲醛浓度呈良好的线性关系,该方法的线性范围为0.02～1.00μg/mL,检出限为1.8×10-3μg/mL,对0.75μg/mL甲醛平行测定11次,其相对标准偏差为2.0%.该方法用于食品中甲醛的测定,回收率为81.2%～87.5%,与乙酰丙酮法对照,结果基本一致.It is found that formaldehyde can react with 1,3-Cyclohexanedione-histamine in the subacidity medium and produce hyperfluorescence componds.Under the optimum conditions,its relative fluorescence intensity is linearly related to the concentration of formaldehyde in the range of 0.02～1.00 μg/mL with a detection limit of 1.8×10-3 μg/mL,the relative standard deviation for the determination of 0.75 μg/mL formaldehyde was 2.0%.The method was applied to determination of formaldehyde in food and the recovery is in the range of 81.2%～87.5%.Compared with acetyacetone method,the results were well.

关 键 词：甲醛 1 3-环己二酮 组胺 荧光光度法 

分 类 号：O657.3[理学—分析化学]