HPLC法测定人血清中奈达铂浓度  

Nedaplatin Determination in Human Serum by HPLC

作　　者：苏华[1] 张文静[1] 

机构地区：[1]山东聊城市第二人民医院药剂科,聊城252601

出　　处：《药学与临床研究》2011年第4期383-384,共2页Pharmaceutical and Clinical Research

摘　　要：目的:建立高效液相色谱法测定奈达铂的血清浓度。方法:采用色谱柱为Spherisorb NH2柱(4.0 mm×300 mm,10μm);流动相:40 mmol·L-1磷酸二氢钾-乙腈(8∶2);流速:0.8 mL·min-1;检测波长:210 nm。结果:奈达铂浓度在5～50μg·mL-1范围内呈良好的线性关系(r=0.9995),奈达铂浓度为5、20、40μg·mL-1时日内和日间平均回收率结果分别为:96.7%、97.2%、97.8%和98.4%、98.2%、99.6%,日内、日间RSD分别为2.09%、1.98%、3.19%和2.27%、2.13%、1.92%(n=5)。结论:该方法具有样品处理简单、稳定性高等特点,适用于奈达铂的药动学研究和治疗药物监测。ABSTRACT Objective： To develop an HPLC method for the determination of nedaplatin concentration in human serum. Methods： A Spherisorb NH2 column was used and a UV detector was set at 210 nm. The mobile phase was a mixture of 40 mmol·L^-1 potassium dihydrogen phosphate solution-acetonitrile （8：2） al a flow rate of 0.8 mL·min^-1. Results： The calibration curve of nedaplatin was linear over the range of 5-50 μg·mL^-1 （r=0.9995）. The intraday and interday average recoveries of the nedaplatin concentrations at 5,20,40 μg·mL^-1 were 96.7%, 97.2%, 97.8% and 98.4%, 98.2%, 99.6% （n=5）, respectively. The RSD were 2,09%, 1.98%, 3.19% and 2.27%, 2.13%, 1.92% （n=5）, respectively. Conclusion： This method is simple, precise, stable and suitable to be applied in the therapeutical drug monitoring and pharmacokinetic studynf nedaplatin.

关 键 词：奈达铂 高效液相色谱法:血药浓度 

分 类 号：R969.1[医药卫生—药理学]