多重分离-光谱法测定饲料中尿素含量  

Quantitate urea within feedstuff by multi-separation and colorimetric spectrometry

作　　者：吴豫鄂[1] 

机构地区：[1]武汉工程大学分析测试中心,湖北武汉430074

出　　处：《武汉工程大学学报》2011年第8期21-24,共4页Journal of Wuhan Institute of Technology

摘　　要：针对饲料这种复杂的体系中基体物质严重干扰其中尿素含量的测定的问题.本研究用活性碳吸附分离色素,乙酸锌和亚铁氰化钾沉淀分离蛋白质、多肽、氨基酸.在优化的分离条件下:样品/活性碳质量比1～2,水/固比10～15,温度40～50℃,pH5,搅拌后静置2 h,再用水淋洗分离,尿素与干扰物的分离率达90%以上.用对二甲氨基苯甲醛光度法对分离后样液中的尿素进行定量测定,尿素回收率达99.9%,RSD为3.0%.Feedstuff was usually complicated mixture.It was difficult to quantitate the urea within it due to severe matrix interference.The present research used activated charcoal to absorb and got rid of pigments,and used zinc acetate and potassium ferrocyanide to precipitate and got rid of proteins,polypeptide and amino acids in feedstuff.The resolution of urea and interference substances were achieved by 90% under optima separation condition as follow： the ratio of sample and activated charcoal was maintained 1 to 2,liquid and solid is maintained 10 to 15,absorb reaction temperature at 40 to 50 ℃ and pH value is adjusted to 5,after stirred 2 hours,let the system stand and undergo adsorption process,then rinsed by distilled water.By above treatment,4-Dimethylaminobenzaldehyde colorimetric spectrometry was employed to quantitate the urea in feedstuff sample,the recovery of urea was 99.9% and RSD was 3.0%.

关 键 词：饲料中的尿素 干扰物的分离方法和分离条件 定量分析 

分 类 号：O652.1[理学—分析化学]