HPLC法测定癸氧喹酯固体分散体有关物质及其含量  

Determination of decoquinate dispersions of solid by HPLC method

作　　者：肖芳[1] 周霁[1] 严少康[1] 黎璐平[1] 刘永琼[1] 

机构地区：[1]武汉工程大学化工与制药学院绿色化工过程省部共建教育部重点实验室湖北省新型反应器与绿色化学工艺重点实验室,湖北武汉430074

出　　处：《武汉工程大学学报》2011年第8期56-59,共4页Journal of Wuhan Institute of Technology

摘　　要：采用高效液相色谱(HPLC)法测定癸氧喹酯固体分散体的有关物质及含量,用C18色谱柱(Dionex5μm,4.6 mm×250 mm),以无水甲醇为流动相,流动相速度为1.0 mL/min,柱温为30℃,检测波长为265 nm.结果发现癸氧喹酯固体分散体在1.0～50 mg/mL浓度范围内呈良好线性关系(R2=0.999 8);平均回收率为101.3%;RSD为0.84%;检测线为0.05 mg/mL.结果表明:本法可快速、准确地测定癸氧喹酯固体分散体的含量和有关物质,适用于产品的质量控制.Established a method for determination of decoquinate disperisions of solid with high performance liquid chromatography（HPLC）.The HPLC method was carried on C18（4.6 mm×250 mm,5 μm） column using anhydrous methanol as mobile phase.The flow rate was 1 mL/min.The column temperature was 30 ℃ and detection wavelength was 265 nm.The result showed the calibration curve for decoquinate dispersions of solid was linear in the range of 1.0-50 mg/mL（R2=0.999 8）.The average recovery of decoquinate dispersions of solid was 101.3%,with RSD 0.84%,the detected limit was 0.05 mg/mL.The method which was rapid,simple,accurate and suitable for the quality control of the drug was found in the research.

关 键 词：高效液相色谱法 癸氧喹酯固体分散体 有关物质 定量分析 

分 类 号：R927.2[医药卫生—药学]