比色法测定黄芪总皂苷的含量  

DETERMINATION OF TOTAL SAPONINS IN ASTRAGALUS MEMBRANACEUS BY COLORIMETRY

作　　者：李春红[1] 田吉[1] 张金平[2] 肖顺汉[1] 

机构地区：[1]泸州医学院药物与功能性食品研究中心,四川泸州646000 [2]泸州医学院基础医学院病原生物学教研室,四川泸州646000

出　　处：《泸州医学院学报》2011年第4期349-351,共3页Journal of Luzhou Medical College

基　　金：四川省教育厅重点项目(2005A76)

摘　　要：目的:建立黄芪总皂苷的含量测定方法。方法:采用比色法测定黄芪总皂苷。结果:总皂苷在0.06～0.6 mg范围内具有良好的线性关系(R=0.9998),回收率为98.25%,RSD为0.44%。结论:该方法操作简便、快速、有效、灵敏、准确、具有良好的重复性和回收率,可作为黄芪总皂苷的含量测定方法。Objective：To establish the determination method of total saponins in astragalus membranaceus.Methods：The content of total saponins was detected by UV.Results：The calibration curve showed good linearity over the range of 0.06～0.6mg（R=0.9998）.The average recovery was 98.25% with RSD 0.44%.Conclusion：This method is simple,rapid,efficient,sensitive and accurate,and has good repeatability and recovery rate.It can be used as a quantitative analysis method for this preparation.

关 键 词：黄芪 总皂苷 比色法 

分 类 号：R927.2[医药卫生—药学]