人脐血CD34^＋细胞体外诱导分化为树突状细胞及其扩增  

Induction and amplification of dendritic cells derived from human cord blood CD34^ ＋ cells in vitro

作　　者：陈东晓[1] 朱远丰[1] 吴林[1] 

机构地区：[1]汕头大学医学院第二附属医院,515041

出　　处：《中国医学创新》2011年第24期19-20,共2页Medical Innovation of China

摘　　要：目的采用脐血CD34^＋细胞在rhGM—CSF、rhTNF—α诱导下体外分化扩增为成熟树突状细胞。方法应用CD34^＋干细胞分选试剂盒以及免疫磁珠法从脐血中分离CD34^＋细胞，用rhGM—CSF、rhTNF—α联合诱导分化发育14d，成为成熟DC，观察细胞形态及检测CD83分子表达。结果50ml脐带血可分离出CD34^＋细胞约1．1×10^6，在rhGM—CSF、rhTNF-α诱导下CD34^＋干细胞培养2周，约扩增10～20倍。CD34^＋干细胞培养第3d开始出现集落，第8～9天集落最大，CD34^＋千细胞分化发育为有树突状突起的成熟DC，表面分子CD83阳性率为81．7％。结论体外联合运用rhGM—CSF、rhTNF—α，能够成功诱导脐血CD34^＋细胞分化为大量成熟的DCs。Objective The proliferation of human dendritic cells （DCs） from cord blood CD34 ^＋ cells induced by rhGM - CSF,rhTNF -α was observed. Methods CD34 ^＋ hematopoietie stem cells were isolated from healthy human cord blood using CD34^ ＋ progenitor cell isolated kit by a high -gradient magnetic cells sorting system（MACS）. The CD34 ^＋ cells were expanded with rhGM - CSF and rhTNF - α for 2 weeks. CD83 was analysed by means of flowcytometry. Results About 1.1×10^6 CD34^ ＋ cells were cultured, expanded and isolated from 50 ml cord blood ： CD34 ^＋ stem cells were cultured in the presence of rhGM - CSF and rhTNF -α, and the cells were expanded 10 - 20 - fold after 2 weeks. In the culture system, the small colony occur in the 3rd day, when expanded, the colony augment gradually and achieve the largest in the 8th to 9th day. About 81.7% expanded cells expessed CD83. Conclusion The in vitro culture system with rhGM - CSF and rhTNF - α can effectively induce cord blood CD34^ ＋ cells to differentiate into dendritic cells.

关 键 词：树突状细胞 脐血CD34^+细胞 rhGM—CSF RHTNF-Α 体外培养 

分 类 号：R329.28[医药卫生—人体解剖和组织胚胎学]