马血清破伤风抗毒素分离纯化的研究  

SEPARATION AND PURIFICATION OF HORSE SERUM TETANUS ANTITOXINS

作　　者：褚洪标[1] 胡有生[1] 彭才圣[1] 梁兆昌[1] 刘永青[1] 

机构地区：[1]井冈山大学医学院,江西吉安343000

出　　处：《井冈山大学学报（自然科学版）》2011年第4期98-100,共3页Journal of Jinggangshan University (Natural Science)

基　　金：吉安市科技局重点科技计划项目(吉市科计字[2009]40)

摘　　要：目的提高马血清破伤风抗毒素制剂中有效成分的含量。方法在超滤浓缩之后,利用凝胶色谱对原液进行纯化,利用电泳图谱对结果进行分析。结果葡聚糖凝胶(Sephadex G-150)对抗毒素的分离有较好效果,可去除一部分杂蛋白,提高F(ab)2等有效成分的含量。结论利用凝胶色谱对抗毒素进行分离可提高制品质量,降低不良反应,因此增加该工艺是可行的。Objective：To improve the content of active constituents in tetanus antitoxins.Methods：After ultrafiltration,the stock solution was purified by gel chromatography method and the results were analyzed by electrophoresis.Results：The contents of F（ab）2 were improved by use of Sephadex G-150 and the impurity proteins were decreased.Conclusion：The product quality was promoted by gel chromatography and the seperation technics may be feasible.

关 键 词：破伤风抗毒素 凝胶色谱 G-150 分离 纯化 

分 类 号：R373[医药卫生—病原生物学]