中草药叶上花黄酮提取方法研究  

Study on Extraction Method of Total Flavonoids in Chinese Herbal Medicine Helwingia Himalaica

作　　者：杨发忠[1] 杨德强[2] 杨斌[3] 李向红[1] 

机构地区：[1]西南林业大学理学院,云南昆明650224 [2]云南师范大学信息学院,云南昆明650092 [3]西南林业大学云南省森林灾害预警与控制重点实验室,云南昆明650224

出　　处：《云南化工》2011年第4期1-4,58,共5页Yunnan Chemical Technology

基　　金：云南省科技计划项目(2008CD140);云南省教育厅基金(08z0027);西南林业大学大学生科技创新项目(1002)

摘　　要：采用定性检测、光谱分析等方法对叶上花总黄酮的种类与提取方法进行了研究。结果显示叶上花所含黄酮种类较多、含量较高;各因素对总黄酮提取率影响程度大小顺序为温度>乙醇体积分数>固液比>时间。最佳提取条件为温度60℃、乙醇体积分数70%、固液比1∶100(g/mL)、提取时间180min,在此提取条件下,总黄酮含量为1.479%。With the methods of the chemical qualitative detection and the spectral analysis,the categories of the total flavonoids in the whole plant of H.himalaica was researched,and effect of four factors（volume fraction of ethanol,temperature,ratio of solid-liquid,extraction time） on the efficiency of extraction of the total flavonoids was also evaluated by orthogonal design L9（34）.The results suggested that the total flavonoids consisted of flavones,flavonols,flavanones,flavanonols.The influencing degree of each factor on the efficiency was as follows： temperaturevolume fraction of ethanolratio of solid-liquidextraction time.The optimum extracting conditions are A1C3B2D3.The highest efficiency of extraction of the total flavonoids reached 1.479% under such optimal conditions.

关 键 词：叶上花 总黄酮 提取方法 

分 类 号：R284[医药卫生—中药学]