有机溶剂对乳化-溶剂挥发法制备PLGA纳米粒的影响  

Influence of organic solvent on preparation of PLGA nanoparticles by emulsificationsolvent evaporation method

作　　者：李晓芳[1] 张晓[1] 陈钢[1] 牧磊[1] 刘素娜[1] 

机构地区：[1]广东药学院药科学院,广东广州510006

出　　处：《广东药学院学报》2011年第4期335-339,共5页Academic Journal of Guangdong College of Pharmacy

基　　金：国家自然科学基金资助项目(30801553);广东药学院师资队伍建设经费资助项目

摘　　要：目的以生物可降解材料聚(乳酸-羟基乙酸)共聚物(PLGA)为载体,采用乳化-溶剂挥发法制备包载荧光标记物香豆素-6的纳米粒,考察有机溶剂组成对纳米粒制备的影响。方法选用不同配比的二氯甲烷与乙酸乙酯混合溶剂作为有机相制备香豆素-6-PLGA纳米粒,测定纳米粒粒径与包封率。结果纳米粒粒径随着乙酸乙酯比例增加而减小,包封率随着二氯甲烷比例增加而递增。二氯甲烷与乙酸乙酯体积比为70∶30时,制得纳米粒的平均粒径为128.7 nm,包封率为35.9%。结论有机溶剂的物理性质对乳化-溶剂挥发法制备PLGA纳米粒的粒径与包封率的影响有统计学意义。采用二氯甲烷与乙酸乙酯体积比为70∶30的混合有机溶剂制得的香豆素-6纳米粒粒度分布较窄,包封率较高。Objective To prepare fluorescence marker coumarin-6 loaded nanoparticles by emulsification solvent evaporation method using the biodegradable material poly（lactic-co-glycolic acid）（PLGA） as carrier,and investigate the influence of organic solvent on nanoparticles′ formation.Methods Coumarin-6 loaded PLGA nanoparticles（coumarin-6-PLGA-NP） were prepared while organic phase was composed of dichloromethane（DCM） and ethyl acetate（EA） at different volume ratio.The entrapment efficiency and particle size of nanoparticles were determined by HPLC method and laser particle size analyzer.Results The nanoparticle size decreased with the increase of ethyl acetate,entrapment efficiency increased with the increase of dichloromethane.The particle size of nanoparticles and the entrapment efficiency was 128.7 nm and 35.9% respectively when combinations of DCM with EA were used at volume ratio of 70 ∶30.Conclusion Physical properties of organic solvents had significant effect on nanoparticles′ formation.PLGA nanoparticles prepared with combination of DCM and EA at ratio of 70 ∶30 exhibit narrow size distribution and higher entrapment efficiency.

关 键 词：聚乳酸- 羟基乙酸共聚物 纳米粒 香豆素-6 乳化-溶剂挥发法 

分 类 号：R94[医药卫生—药剂学]