HPLC-ELSD法测定化橘红中肌醇含量  

Determination of the content of inositol in Citri Grandis by HPLC-ELSD

作　　者：陈地灵[1] 陈振兴[1] 林励[1] 王莲婧[1] 帅欧[1] 庄满贤[1] 

机构地区：[1]广州中医药大学中药学院,广东广州510006

出　　处：《广东药学院学报》2011年第4期385-387,共3页Academic Journal of Guangdong College of Pharmacy

基　　金：国家科技部"十二五"科技支撑计划项目(2011BAI01B02);国家发改委高技专项"名贵道地中药材化橘红产业化示范基地"(2003-26)

摘　　要：目的建立化橘红中肌醇含量测定方法。方法采用高效液相色谱-蒸发光散射检测器（HPLC-ELSD）法进行测定,色谱柱为Rezex RCM-Monosaccharide Ca2＋（8%）（Phenomenex,300 mm×7.8 mm）;流动相为去离子水,流速为0.45 mL.min-1;飘移管温度100℃;雾化气体为空气,流速为2.0 L·min-1;柱温为35℃。结果肌醇在0.25~8.00 mg·mL-1范围内线性关系良好,r=0.999 8,平均回收率为99.32%,RSD=3.34%,样品中其他成分不干扰测定。结论本法简便、准确、可靠,可用于化橘红中肌醇含量的测定。Objective To establish a method for determining the content of inostiol in Citri Grandis.Methods The content was determined by high performance liquid chromatography coupled with evaporative light-scattering detector（HPLC-ELSD） method.The operation was performed on a Rezex RCM-Monosaccharide Ca2＋（8%）cloumn（Phenomenex,300 mm×7.8 mm） using deionized water as the mobile phase at the flow rate of 0.45 mL·min-1.The temperature of drift tube and the column was set at 100 ℃ and 35 ℃,respectively.The gas flow rate was 2.0 L·min-1.Results The calibration curve of inositol displayed ideal linearity over the range of 0.25-8.00 mg·mL-1（r=0.999 8）,and the average recovery was 99.32% with RSD of 3.34%.The other ingredients in the sample showed no interference.Conclusion The method established could be used for determination of inostiol in Citri Grandis due to its convenience,good reproducibility and high accuracy.

关 键 词：肌醇 化橘红 HPLC-ELSD法 含量测定 

分 类 号：R284.1[医药卫生—中药学]