壁虎醇提物对人食管癌EC-109细胞增殖抑制作用及其机制研究  

Inhibitory Effects of Gecko Alcohol Extract on Human Esophageal Squamous Carcinoma Cell Line EC-109 Proliferation and Associated Mechanism

作　　者：宋佳玉[1,2] 王晓兰[1] 王建刚[1] 席守民[1] 刘玲[1] 李瑞芳[1] 余凯[1] 

机构地区：[1]河南科技大学医学院,河南洛阳471003 [2]河南漯河医学高等专科学校,河南漯河462002

出　　处：《中药材》2011年第7期1020-1023,共4页Journal of Chinese Medicinal Materials

基　　金：河南省医学科技攻关计划项目(200903109)

摘　　要：目的:研究壁虎醇提物(Gecko alcohol extract,GAE)对人食管癌EC-109细胞的增殖抑制作用及其机制。方法:MTT法检测GAE对细胞增殖抑制作用;Hoechest 33342荧光染色观察细胞形态学的改变;流式细胞术检测凋亡率;SP免疫组化法检测Fas和Caspase-3蛋白的表达。结果:GAE具有抑制食管癌EC-109细胞增殖的作用;3.0mg/mL、4.0 mg/mL GAE作用细胞48 h后凋亡率分别为20.60%和37.93%;与正常对照组相比GAE组Fas蛋白和Caspase-3蛋白的表达显著增加(P<0.05)。结论:GAE能够抑制EC-109细胞增殖并诱导其凋亡,其作用机制可能与增加Fas蛋白和Caspase3蛋白的表达有关。Objective：To explore the proliferation inhibition effects of Gecko alcohol extract（GAE） on human esophageal squamous carcinoma cell line EC-109 and its mechanism.Methods： The inhibitory effects of GAE on proliferation of EC-109 cells were measured by MTT.Nucleolus change of apoptotic cells was observed by Hoechest33342 fluorescence staining.Apoptosis rate of EC-109 cells was detected by flow cytometry.The expressions of apoptosis protein Caspase-3 and FAS in EC-109 cells were investigated by immunohistochemistry.Results： GAE had the inhibition effects on the proliferation of esophageal carcinoma cell EC-109.The apoptosis rate of EC-109 cell treated with GAE（3.0 mg/mL,4.0 mg/mL）for 48h was 20.63% and 39.73%,respectively.Compared with control group,the expression of Fas and Caspase-3 was significantly up-regulated in GAE treated group. Conclusion： GAE can inhibit the proliferation of esophageal carcinoma EC-109 cells and induce them apoptosis which may be correlated with increasing expression of protein Fas and Caspase-3.

关 键 词：壁虎醇提物 细胞凋亡 食管癌 CASPASE-3 FAS 

分 类 号：R285.5[医药卫生—中药学]