不同生长年限太白贝母中贝母辛和总生物碱的含量测定  

Quantitative Determination of Peimisin and Total Alkaloids in Fritillaria taipaiensis of Different Growing Stage

作　　者：王怀玉[1] 马鹏[2] 彭锐[2] 

机构地区：[1]绵阳师范学院,四川绵阳621000 [2]重庆市中药研究院,重庆400065

出　　处：《中药材》2011年第7期1034-1037,共4页Journal of Chinese Medicinal Materials

基　　金：重庆市卫生局资助项目(08-1-177)

摘　　要：目的:测定不同生长年限太白贝母鳞茎中贝母辛和总生物碱的含量,为建立和完善中药材太白贝母的质量控制体系和适宜采收时间提供实验依据。方法:采用HPLC-ELSD法测定贝母辛,色谱柱为Agilent Extend C18(250 mm×4.0 mm,5μm),流动相为乙腈-0.1%二乙胺水溶液(10∶90),流速为1.0 mL/min,Alltech 2000蒸发光散射检测器;采用紫外分光光度法测定总生物碱。结果:生长2～3年的太白贝母贝母辛的含量随着生长年限的增加而增加,生长4～6年的太白贝母贝母辛的含量随着生长年限的增加而降低;生长2～4年太白贝母的总生物碱含量逐年增加,生长5～6年总生物碱含量逐年下降。结论:综合药用有效成分贝母辛和总生物碱的含量、贝母药材的产量以及土地利用率3个因素考虑,以4年生的太白贝母采收比较合适。Objective： To study the content of peimisine and total alkaloids in Fritllaria taipaiensis of different growing stage. Methods： High performance liquid chromatography coupled with evaporative light scattering detector was employed,and peimisine was separated and quantified upon an Agilent Extend C18（250 mm×4.0 mm,5μm）,using acetonitrile-water-diethylamine as the mobile phase at the flow rate of 1.0 mL·min-1;UV spectrophotometry was used for the determination of total alkaloids.Results： During from two to three years period,the content of peimisine in F.taipaiensis increased with growing stage,from four to six years,it decreased with growing stage;From two to four years,the content of total alkaloids in F.taipaiensis increased with growing stage,from five to six years,it decreased with growing stage.Conclusion： It is suitable to harvest F.taipaienssi of four years growing stage.

关 键 词：太白贝母 贝母辛 总生物碱 高效液相色谱-蒸发光检测法 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]