高速逆流色谱法分离岩黄连中的生物碱类成分  

Isolation of Alkaloids from Corydalis saxicola by High-speed Counter-current Chromatography

作　　者：程轩轩[1] 杨得坡[2] 王冬梅[2] 蒋林[2] 

机构地区：[1]广东药学院中药学院,广东广州510006 [2]中山大学药学院,广东广州510006

出　　处：《中药材》2011年第7期1062-1064,共3页Journal of Chinese Medicinal Materials

摘　　要：目的:从岩黄连的粗提物中快速分离生物碱类成分。方法:采用高速逆流色谱技术,以正丁醇-乙酸乙酯-水-甲酸(5∶1∶5∶0.01)和正丁醇-乙酸乙酯-甲醇-水-甲酸(5∶5∶1∶9∶0.05)作为两相溶剂系统分离岩黄连中的主要生物碱。结果:经过连续三次色谱分离,从岩黄连的正丁醇粗提物(300 mg)中得到6个纯度较高的生物碱,分别为:scoulerine(3.6 mg,质量分数:71%)、isocorydine(9.2 mg,质量分数:92%)、dehydrocheilanthifoline(5.5 mg,质量分数:85%)、dehydrocavidine(7.5 mg,质量分数:76%)、palmatine(20.4 mg,质量分数:90%)、berberine(20.9 mg,质量分数:97%)。结论:采用高速逆流色谱法制备分离岩黄连中的生物碱,省时、方便、产物得率和纯度较高。Objective：To develop a method for the rapid isolation of alkaloids from the crude extract of Corydalis saxicola.Methods：High-speed counter-current chromatography（HSCCC） was applied for the separation of alkaloids from Corydalis saxicola,with the biphase solvent systems comprised n-butanol-ethyl acetate-water-formic acid（5∶1∶5∶0.01） and n-butanol-ethyl acetate-methanol-water-formic acid（5∶5∶1∶9∶0.05）.Results：About 300 mg of crude extract was isolated by HSCCC,yielding 3.6 mg of scoulerine,9.2 mg of isocorydine,5.5 mg of dehydrocheilanthifoline,7.5 mg of dehydrocavidine,20.4 mg of palmatine and 20.9 mg of berberine,with purities of 71%,92%,85%,76%,90%,97%,respectively.Conclusion：It is time-saving and simple to isolate alkaloids from Corydalis saxicola by HSCCC with high yields and purities.

关 键 词：高速逆流色谱 岩黄连 生物碱 

分 类 号：R284.1[医药卫生—中药学] R284.2[医药卫生—中医学]