塔斯品碱衍生物对人肝癌SMMC7721细胞生长的抑制作用研究  

Effect of Taspine Derivatives on Human Liver Cancer SMMC7721

作　　者：张彦民[1] 王楠[1] 代秉玲[1] 贺浪冲[1] 

机构地区：[1]西安交通大学医学院,陕西西安710061

出　　处：《中药材》2011年第7期1094-1097,共4页Journal of Chinese Medicinal Materials

基　　金：国家自然科学基金面上项目(81001447);陕西省自然科学基金(2010JQ4017)

摘　　要：目的:分析探讨塔斯品碱衍生物对人肝癌SMMC7721细胞的生长抑制作用,研究其作用机制。方法:采用MTT法测定5种塔斯品碱衍生物对肝癌SMMC7721细胞生长的抑制作用,流式细胞术检测Tas-D1对肝癌SMMC7721细胞周期的影响,ELISA法测定Tas-D1对肝癌SMMC7721细胞EGF和VEGF蛋白的影响,PCR法测定Tas-D1对EGF和VEGF的mRNA水平的影响。结果:塔斯品碱衍生物Tas-D1对SMMC7721细胞生长具有明显的抑制作用,使细胞阻滞在S期,呈现出良好的剂量依赖性。ELISA法和RT-PCR法结果表明Tas-D1对SMMC7721细胞的EGF和VEGF蛋白质的表达和mRNA的表达有一定的抑制作用(P<0.05)。结论:塔斯品碱衍生物Tas-D1能抑制人肝癌SMMC7721细胞的生长,转换细胞周期,初步作用机制主要为Tas-D1抑制SMMC7721细胞EGF和VEGF的表达。Objective：To analyse the inhibition effect of taspine derivatives on human Liver cancer SMMC7721 cell and its mechanism.Methods：The effects of five taspine derivatives on SMMC7721 cell growth were determined by MTT.The flow cytometry was used to determine the cell cycle.The effects of Tas-D1 on the EGF and VEGF in SMMC7721 cell were determined by ELISA.The mRNA level of EGF and VEGF in SMMC7721 cell was determined by RT-PCR.Results：The MTT assay demonstrated that the taspine derivative Tas-D1 significantly inhibited the growth of SMMC7721 cell in a dose-dependent manner.Cell was stopped at S phase by Tas-D1.Tas-D1 inhibited the expression of EGF and VEGF and their mRNA in a dose-dependent manner（P0.05）.Conclusions：The taspine derivative Tas-D1 can inhibit the growth of human Liver cancer SMMC7721 cell and change cell cycle,which may be related to the inhibition of EGF and VEGF expression.

关 键 词：塔斯品碱衍生物 SMMC7721细胞 生长抑制 表达 

分 类 号：R285.5[医药卫生—中药学]