HPLC法检测水产品中孔雀石绿、结晶紫及其关联化合物的残留  

Determination of malachite green,crystal violet and their leuco-metabolites in aquatic product by HLPC

作　　者：白丽丽[1] 林正峰[2] 戴华[3] 

机构地区：[1]海南医学院药学院,海南海口571101 [2]海南出入境检验检疫局,海南海口570311 [3]海南医学院公共卫生学院,海南海口571101

出　　处：《海南医学院学报》2011年第9期1157-1159,1163,共4页Journal of Hainan Medical University

基　　金：海南医学院科研基金资助学报项目(0020110302)~~

摘　　要：目的:建立高效液相色谱法检测虾类水产品中孔雀石绿、结晶紫及其相关代谢物残留量的方法。方法:样品经乙腈提取后、浓缩、纯化,经Waters UPLC BEH C18柱(2.1 mm×50 mm,粒径1.7μm)分离,以0.5 mmol/L乙酸胺∶乙腈=30∶70为流动相进行测定分析。结果:孔雀石绿、无色孔雀石绿的加标平均回收率分别为:90.30%～102.83%、85.10%～98.60%,RSD分别为:4.96%～6.88%、6.24%～11.65%。结论:本方法操作简单,回收率稳定,灵敏度高,适用于水产品中的孔雀石绿、无色孔雀石绿残留量的检测。Objective： To establish a credible analytical method based on high performance liquid chromatography for the determination of malachite green（MG）,crystal violet and their leuco-metabolites in aquatic product.Methods： The MG,crystal violet and their leuco-metabolites were extracted by acetonitrile,then were concentrated and purified.Samples were isolated by Waters UPLC BEH C18 column（2.1 mm×50 mm,diameter 1.7 μm）and determined with 0.5 mmol/L ammonium acetate∶acetonitrile（30∶70） as mobile phase.Results： The mean recovery rate of MG and leucomalachite green were in the range of 90.3%-102.83% and 85.10%-98.60%,respectively.Values of RSD were 4.96%-6.88% and 6.24%-11.65%,respectively.Conclusions： The method is simple,sensitive,stable and suitable for the determination of trace malachite green and leucomalachite green green in aquatic product.

关 键 词：高效液相法 孔雀石绿 结晶紫 

分 类 号：R971[医药卫生—药品]