板栗花中总多酚提取工艺优化  

Process Optimization for Extraction of Total Polyphenols from Chinese Chestnut Flowers

作　　者：杜彬[1] 王同坤[1] 侯文龙[1] 牛少莉[1] 杨越冬[1] 

机构地区：[1]河北科技师范学院分析测试中心,河北秦皇岛066004

出　　处：《食品科学》2011年第16期121-126,共6页Food Science

基　　金：河北省科技支撑计划项目(09231005D);河北省重点基础研究项目(09960912D);河北省自然科学基金项目(C2010001523)

摘　　要：研究板栗花中多酚类物质的最佳提取工艺条件。采用传统回流加热法对板栗花中多酚类物质进行提取试验研究,通过4种不同试剂在相同实验条件下的浸提效果进行比较分析,确定最佳提取试剂为甲醇。然后分别对微波浸提法和超声波浸提法提取板栗花中多酚类物质的工艺进行比较研究,通过对甲醇体积分数、浸提时间、料液比等单因素的浸提效果进行研究,确定最佳单因素水平,再通过正交试验分别确定以甲醇为浸提剂提取板栗花中多酚类化合物的最佳提取工艺条件。通过验证实验确定最佳提取方法为微波浸提法,最后提取条件为40%甲醇溶液、提取时间60s、微波功率480W、料液比1:25(g/mL),在此条件下,多酚得率为6.69%。In this study,the optimal extraction conditions of total polyphenols from the flowers of Chinese chestnut cultivar Yanlong were investigated.The traditional heating reflux method was used for the extraction of total polyphenols.Four different extraction solvents were applied for the extraction of polyphenols.The results indicated that methanol was the best extraction solvent,and microwave was superior to ultrasonic in enhancing the extraction of total polyphenols.The optimal process parameters for microwave-assisted extraction of total polyphenols were determined by orthogonal array design to be 40% methanol as the extraction solvent,microwave treatment time of 60 s,microwave power of 480 W and material-to-liquid ratio of 1：25（g/mL）.Under the optimal extraction conditions,the extraction rate of total polyphenols from chestnut flowers was up to 6.69%.

关 键 词：板栗花 总多酚 提取工艺 优化 

分 类 号：TQ243.11[化学工程—有机化工]