丙泊酚对内毒素性急性肺损伤大鼠血清TNF-α、IL-1β及IL-10的影响  

Propofol attenuates the level of TNF-α,IL-1β and IL-10 in LPS-induced acute lung injury in rats

作　　者：陈龙 朱煊 陈西艳 房孝梅 张茂银[2] 刘功俭[2] 

机构地区：[1]徐州市第六人民医院麻醉科,221006 [2]徐州医学院附属医院麻醉科

出　　处：《临床麻醉学杂志》2011年第8期815-817,共3页Journal of Clinical Anesthesiology

基　　金：江苏省"六大人才高峰"基金项目(06-B-065);江苏省铜山县科技计划项目(TS005)

摘　　要：目的探讨内毒素性急性肺损伤(ALI)大鼠用丙泊酚后处理对血清肿瘤坏死因子(TNF)-α、白细胞介素(IL)-1β和IL-10影响。方法雄性SD大鼠96只,随机均分为四组,每组24只。脂多糖(LPS)致伤组(B组)、丙泊酚低剂量治疗组(C组)和丙泊酚高剂量治疗组(D组)经尾静脉注射LPS 5 mg/kg制作ALI模型;之后生理盐水对照组(A组)和B组泵入生理盐水,C、D组分别注射丙泊酚2、4 mg/kg后再分别泵入4、8 mg.kg-1.h-1。分别在制模后1、2、3、4 h抽取动脉血测定TNF-α、IL-1β及IL-10的水平。结果制模后各时点A组大鼠TNF-α、IL-1β和IL-10含量均明显低于其它三组(P<0.05或P<0.01)。制模后1、2 h B组TNF-α、IL-1β和IL-10含量明显高于C、D组(P<0.01),C组IL-1β和IL-10含量明显高于D组(P<0.05或P<0.01)。制模后3、4 h B组IL-1β和IL-10含量仍明显高于C、D组(P<0.01),C组显著高于D组(P<0.01)。结论丙泊酚可以显著抑制ALI大鼠血清TNF-α、IL-1β及IL-10上升的程度。Objective To investigate the effects of propofol aftertreatment on serum production of TNF-α, IL-1β and IL-10 in lipopolysaccharide-induced acute lung injury rats. Methods Ninty-six male SD rats were randomly divided into 4 equal groups （n= 24）： control group （group A）; LPS group（group B） ; LPS＋ low dose of propofol group（group C） ; LPS＋ large dose of propofol group （group D）. 5 mg/kg LPS was infused through the vena caudalis to produce ALI model. In group C and D, 2 and 4 mg/kg propofol was infused respectively, maintained with 4 or 8 mg· kg^-1 · h^-1. 0. 9% saline was administered in group A and B. The samples of arterial blood were collected to measure the level of TNF-α, IL-1β and IL-10 at the time of 1 h, 2 h, 3 h and 4 h after LPS infused （saline in group A）. Results The level of TNF-α, IL-1β and IL-10 in group A was significantly lower than that in other groups at all time points （P〈0.05 or P〈0.01）. In group C and D, the production of TNF-α, IL-1β and IL-10 was obviously lower than that in group B 1 h or 2 h after LPS administration（P〈0. 01）. At the time of 3 and 4hr after LPS infused, IL-β and IL-10 expression in group C and D was significantly lower than that in group B （P〈0.01）. Compared with group C, the IL-1β and IL-10 level in group D decreased significantly at all time points （P〈0. 05 or P〈0. 01）. Conclusion Treatment with propofol can significantly inhibit the increase of TNF-α, IL-1β and II;10 after LPS-induced acute lung injury in rats.

关 键 词：丙泊酚 内毒素 急性肺损伤 肿瘤坏死因子α 白细胞介素-1Β 白细胞介素-10 

分 类 号：R965[医药卫生—药理学]