水稻细菌性条斑病菌遗传多样性和致病型分化研究  

Genetic Diversity and Pathotype Variability of Xanthomans oryzae pv.oryzicola Strains

作　　者：张荣胜[1,2] 陈志谊[1] 刘永锋[1] 

机构地区：[1]江苏省农业科学院植物保护研究所,江苏南京210014 [2]南京农业大学植物保护学院,江苏南京210095

出　　处：《中国水稻科学》2011年第5期523-528,共6页Chinese Journal of Rice Science

基　　金：农业部公益性(农业)行业科研专项(nyhyzx07-056);国家863计划现代农业技术领域重大项目(2006AA10A211);江苏省自然科学基金资助项目(BK2008350)

摘　　要：通过rep-PCR指纹技术,分析了来源江苏、云南、江西、湖南和安徽等省69个水稻细菌性条斑病菌(Xanthomonasoryzae pv.oryzicola)群体遗传多样性。用2对特异性引物BOX和ERIC对菌株基因组DNA进行了PCR扩增,以彼此间的带位相似率达80%为界,根据BOX扩增的谱型将供试菌株分为10类,根据ERIC的谱型则分为8类。BOX的第3类包含27个菌株,占总数的39.1%,ERIC的第5类包含24个菌株,占总数的34.8%,均为优势群。群体遗传多样性值分别为0.9784(BOX)和0.9833(ERIC)。说明水稻细菌性条斑病菌群体的遗传分化明显,遗传多样性较高。全部69株菌株接种于含有不同抗病基因近等基因系及高感品种金刚30等6个鉴别品种上,被划分为13个致病型。其中,C6致病型占26.1%,为优势致病型。69个菌株根据BOX、ERIC扩增结果进行的类群分类与地理位置显著相关,与致病型分化相关不显著。Genetic diversity and pathotype variability of Xanthomans oryzae pv. oryzicola strains collected from Jiangsu, Yunnan, Jiangxi, Hunan and Anhui Provinces were analyzed by using rep PCR fingerprinting techniques. Genomie DNAs from 69 strains were amplified with two specific primers BOX and ERIC. Dendrograms were generated from the data by using UPGMA analysis. The tested strains were classified into 10 and8 groups at a similarity of 80%o based on the amplification results with BOX and ERIC, respectively. The predominant groups were group 3 and C-roup 5 for the primers BOX and ERIC, involving 27（39.1%） and 24（34.8%） strains, respectively. The genetic diversities of the population of the tested strains were 0.9784 （with BOX） and 0. 9833 （with ERIC）, respectively, indicating the high genetic diversity of Xanlhornans oryzae pv. oryzicola. According to inoculation results on five differential cultivars including nea〉isogenic rice lines carrying single resistance genc, IRBB4, IRBBS, IRBB14, IRBB21 and IRBB24, and a highly susceptible cultivar Jingang 30, the 69 strains were classified into 13 pat hotypes. The predominant pathotype was C6 （26.1%）. No relationships were observed between UPGMA groups （BOX and ERIC） of the 69 strains and pathotypes, but each group was partially associated with the regional origin of strains.

关 键 词：REP-PCR 水稻细菌条斑病菌 遗传多样性 致病型 

分 类 号：S435.111[农业科学—农业昆虫与害虫防治]