Ⅰ型胶原凝胶包埋脂肪干细胞复合PLGA-β-TCP支架修复兔自体桡骨缺损  

Repair of rabbit segmental radial defect by using collagen Ⅰgel to suspend autologous adipose-de- rived stem cells into porous PLGA-β-TCP scaffold

作　　者：郝伟[1] 姜明[2] 王新[1] 刘洪智[1] 周路纲[1] 孙煜杰[1] 胡蕴玉[3] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院创伤骨科,烟台264000 [2]济南军区总医院口腔科 [3]第四军医大学西京医院全军骨科研究所

出　　处：《中华实验外科杂志》2011年第9期1547-1549,F0004,共4页Chinese Journal of Experimental Surgery

基　　金：全军医药卫生科研基金资助项目（100211）

摘　　要：目的采用Ⅰ型胶原凝胶悬浮包埋兔脂肪干细胞并复合PLGA-β-TCP支架修复自体桡骨缺损。方法使用Ⅰ型胶原凝胶悬浮兔脂肪干细胞并与PLGA-β-TCP复合构建脂肪干细胞一Ⅰ型胶原凝胶／PLGA-β-TCP复合体（ASCs—COL／PLGA-β-TCP）（A组），同时设立单纯细胞／PLGA-β-TCP材料复合体（ASCs／PLGA-β-TCP）（B组）、单纯Ⅰ型胶原凝胶／PLGA-β-TCP复合体（COIMPLGA-β-TCP）（C组）、单纯PLGA-β-TCP支架材料（D组）以及空白缺损（E组）作为对照，体外成骨诱导培养2周后植入桡骨1．5cm缺损部位。30只兔（60侧）采用两因素随机区组设计每只兔两侧骨缺损植入组别。分别于术后8、16、24周处死兔，取材，进行相关分析。结果术后8周，大体观察、放射学、组织学检测示A组桡骨断端连续性基本恢复。术后16周，A组骨断端连续性完全恢复，髓腔未通。术后24周，A组髓腔再通，改建塑形趋于完成，材料基本降解。自术后16～24周，A组残存材料比例低于其他3组（P〈0．05，n=4）；与A组比较，在整个观察周期内，B、C、D组均无明显成骨现象，E组保持骨缺损状态（P〈0．05，n=4）。结论通过应用Ⅰ型胶原凝胶悬浮包埋脂肪于细胞进而与PLGA-β-TCP多孔支架材料复合构建新型仿生骨组织工程复合体，可修复兔桡骨1．5cm缺损。Objective To repair segmental radial defect by using collagen Ⅰ gel to suspend rabbit adipose-derived stem cells （ASCs） into a porous PLGA-β-TCP scaffold （ASCs-COL/PLGA-β-TCP）. Methods 1.5 cm segmental radial defects were created bilaterally in 30 rabbits. After being cultured in vitro for 2 weeks under osteogenic medium, ASCs-COL/PLGA-β-TCP composite （group A）, the single combination of ASCs and PLGA-β-TCP （ASCs/PLGA-β-TCP） （group B）, combination of acellular collagen I gel and PLGA-β-TCP （COL/PLGA-β-TCP） （group C）, and PLGA-β-TCP scaffold （group D） were transplanted inot the untreated radial defect as blank control group （group E）. Eight, 16, 24 weeks after implantation, 10 animals were sacrificed and the constructs were harvested and analyzed. Results In group A, bone integrity was almost finished 8 weeks postoperation, bone integrity was reconstructed but bone marrow was not recanalized 16 weeks postoperation, bone integrity was completed and bone marrow was recanalized 8 weeks later. Compared with group A, no sign of bone formation was observed in groups B, C, D during the whole period （P 〈 0. 05, n = 4）. Histomorphometric analyses indicated that the new bone forming area as well as scaffold degradation rate in group A were significantly increased as compared with groups B, C and D （ P 〈 0. 05, n = 4）. Conclusion The ASCs-COL/PLGA-β-TCP composite exhibits a favorable osteogenesis, biocompatibility and biodegradability during the repair of autologous segmental radial defect model and therefore cast a new light on the fabrication of cell-based bone tissue engineering constructs.

关 键 词：脂肪干细胞 Ⅰ型胶原 骨组织工程 桡骨缺损 

分 类 号：R318.08[医药卫生—生物医学工程]