正常髓核细胞的黏弹性研究  

Viscoelastic properties of human normal nucleus pulposus cells

作　　者：任龙韬[1] 卫陈刚[2] 牛建鹏[1] 郭志坚[1] 郝海虎[1] 

机构地区：[1]太原市中心医院骨科,030009 [2]山西医科大学研究生

出　　处：《中华实验外科杂志》2011年第9期1559-1560,共2页Chinese Journal of Experimental Surgery

摘　　要：目的观察正常髓核细胞的黏弹性。方法髓核组织取材于3例脊柱侧凸矫形手术者术中取出废弃的髓核组织，用胰蛋白酶和Ⅱ型胶原酶消化分离细胞，Ⅱ型胶原免疫荧光组化和蕃红染色进行细胞鉴定，测量细胞直径，采用微管吸吮技术分析髓核细胞的黏弹性特性。结果髓核细胞直径为（15．40±1．83）μm，正常髓核细胞的黏弹性参数k1（0．101±0．052）kPa、k2（0．353±0．199）kPa和μ（3．034±1．843）kPa·S。直线相关性分析表明，仅k1与髓核细胞直径明显相关（r=-0．389，P〈0．05）。结论正常髓核细胞表现为典型的黏弹性固体蠕变特征；微管吸吮技术可以作为测量髓核细胞生物力学特性的可靠方法。Objective To study the viscoelastic properties of nucleus pulposus （NP） cells from human in vitro. Methods NP was obtained from discarded NP tissue of 3 scoliosis patients aged from 13 to 16 years. Pancreatin and eollagenase type Ⅱ were used to digest NP and cells were isolated from NP. Type II collagen immunofluorescence and Fan seaing were used to identify NP cells. The micropipette aspiration test was used in combination with a three-parameter viscoelastic solid model to measure the mechanical properties of NP cells. Results The mean diameter of the digested NP cells was （ 15.40 ± 1.83 ） p.m. In response to a prescribed pressure, the NP cells exhibited viscolastie solid creep behavior, which was characterized initially by a jump in displacement followed by a monotony decreasing rate of deformation that generally reached an equilibrium. NP cells were deformed to a length as much as 2 times the radius of the micropipette without completely entering the mieropipette. The viscolastie parameters were k1 （0. 101 ± 0. 052） kPa, k2（0. 353±0. 199） kPa, and μ （3. 034 ± 1. 843） kPa-s, respectively. Only the kt was positively correlated to the cell diameter （ r = - 0. 389, P 〈 0. 05 ）. Conclusion Human normal NP cells behave as a typical viscolastic solid creep. Mieropipette aspiration technique is a valid method for the study on biomeehanics of NP cells.

关 键 词：髓核细胞 生物力学 黏弹性 

分 类 号：R687.3[医药卫生—骨科学]