棉粕非淀粉多糖的体外酶解效果研究  

Effect of Enzymatic Hydrolysis in vitro for Non-starch Polysaccharides in Cottonseed Meal

作　　者：王洁[1,2] 李铁军[2] 崔志杰[1] 易克[1] 方俊[1] 

机构地区：[1]湖南农业大学生物科学技术学院,湖南长沙410128 [2]中国科学院亚热带农业生态研究所,湖南长沙410125

出　　处：《湖南农业科学》2011年第8期13-15,共3页Hunan Agricultural Sciences

基　　金：中国科学院绿色农业技术集成与发展中心创新方向项目资助(XMXX281177);武汉新华扬生物股份有限公司合作项目

摘　　要：采用单因子设计,在一定水分(料水比为1∶10)、温度(40℃)和pH值(6.0)条件下酶解2 h时棉粕非淀粉多糖含量的变化,对非淀粉多糖酶对棉粕非淀粉多糖的降解效果进行了研究。结果表明:木聚糖酶酶浓度为4 000 U/kg时,对可溶性和不可溶nsp的降解效果都是最大;甘露聚糖酶酶浓度为4 000 U/kg时,对可溶性nsp的降解效果最明显,当酶浓度为5 000 U/kg时,对不可溶nsp的降解效果最明显。两种nsp酶对棉粕都有明显降解作用。Using single factor design to study the hydrolysis effect of non-starch polysaccharides （nsp） enzyme on nsp in cottonseed meal under the same conditions with the conditions making nsp content in wheat changed, which are as follows： ratio of material to water was 1：10, temperature was 40℃, pH value was 6.0 and hydrolysis time was 2 h. The results indicated that when concentration of xylanase was 4 000 U/kg, the hydrolysis effects xylanase on soluble nsp and insoluble nsp were the highest; when concentration of mannase was 4 000 U/kg, the hydrolysis effect of mannase on soluble nsp of was the most obvious; when concentration of mannase was 5 000 U/kg, the hydrolysis effect of mannase on insoluble nsp was the most obvious; both kinds of nsp enzyme all had obvious hydrolysis effect on cottonseed meal.

关 键 词：非淀粉多糖 酶 棉粕 

分 类 号：S562[农业科学—作物学]