基于代谢组学的脾气虚证本质研究  

Study on the Nature of Spleen Qi Deficiency Based on Metabonomics

作　　者：战丽彬[1,2] 初艳 赵欣捷[4] 许国旺[4] 张丽 隋华[2] 刘颖[1] 吴本慧[1] 

机构地区：[1]大连医科大学附属第二医院,大连116023 [2]大连医科大学中西医结合研究院,大连116044 [3]大连市旅顺第二人民医院,大连116041 [4]中国科学院大连化学物理研究所,大连116023 [5]解放军403医院,大连116001

出　　处：《世界科学技术-中医药现代化》2011年第4期622-625,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：辽宁省教育厅创新团队项目资助(2008T027):基于代谢组学的脾气虚证本质研究;负责人:战丽彬

摘　　要：目的:通过分析血浆小分子代谢组成分,发现脾气虚证代谢综合征组不同于其它组的血浆代谢图谱,并发现脾气虚证代谢综合征的生物标志物,从而揭示中医脾气虚证本质,为临床诊断和治疗脾气虚证提供客观依据和指导。方法:通过收集体检者的基本资料、结合代谢综合征及脾气虚证诊断标准,将受试人员分为正常对照组、代谢综合征组和脾气虚证代谢综合征组;由超高效液相色谱-四极杆-飞行时间质谱联用技术(UPLC-Q-TOF-MS)分离血浆代谢产物。结果:3组血浆代谢产物得到良好区分;代谢综合征组中lysoPC C16∶0、GPCho(32∶2)、GPCho(32∶1)比正常组浓度明显升高(P<0.05);脾气虚证代谢综合征组中carnitine C8∶0、GPCho(38∶6)和分子量341.282的未知化合物比代谢综合征组的浓度明显降低(P<0.05)。结论:脾气虚证代谢综合征组呈现出不同于正常对照组和代谢综合征组的代谢图谱;发现3种化合物可能为代谢综合征的潜在标志物,3种化合物可能为脾气虚证代谢综合征的潜在标志物。This study aimed to analyze small molecular metabolic in plasma in order to find the spectra of plasma in metabolic syndromes （MS） of spleen qi deficiency, which is different from others. Basic documents of this subject were collected in combination with the diagnostic criteria of metabolic syndrome and spleen qi deficiency. UPLC-Q-TOF-MS was used in the separation of plasma metabolites. The results showed that the spectrum of plasma in MS of spleen qi deficiency is different from healthy subjects in metabonomics （P〈 0.05）. It is concluded that three compounds are possibly the latent markers of MS. And another three compounds are possibly the latent markers of spleen qi deficiency.

关 键 词：脾气虚 代谢综合征 证病结合 血浆 代谢组学 

分 类 号：R256.3[医药卫生—中医内科学]